Isolation and identification of Streptomyces sp. Act4Zk, a good producer of Staurosporine and some derivatives
© 2020 The Society for Applied Microbiology..
In this study, strain Streptomyces sp. Act4Zk was isolated based on a method developed for the isolation of myxobacteria. Due to the low efficiency of the majority of conventional DNA extraction techniques, for molecular identification of the strain Streptomyces sp. Act4Zk, a new technique for DNA extraction of Actinobacteria was developed. In order to explore potential bioactivities of the strain, extracts of the fermented broth culture were prepared by an organic solvent (i.e. ethyl acetate) extraction method using. These ethyl acetate extracts were subjected to HPLC fractionation against standard micro-organisms, followed by LC/MS analysis. Based on morphological, physiological, biochemical and 16S rRNA gene sequence data, strain Streptomyces sp. Act4Zk is likely to be a new species of Streptomyces, close to Streptomyces genecies and Streptomyces roseolilacinus. Antimicrobial assay indicated high antifungal activity as well as antibacterial activity against Mycobacterium smegmatis and Gram-positive bacteria for the new strain. HPLC and LC/MS analyses of the extracts led to the identification of three different compounds and confirmed our hypothesis that the interesting species of the genus Streptomyces being a good producer of staurosporine and some derivatives.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
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| Enthalten in: |
Letters in applied microbiology - 72(2021), 2 vom: 21. Feb., Seite 206-218 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khosravi Babadi, Z [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.03.2021 Date Revised 16.03.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/lam.13415 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM316278335 |
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| 245 | 1 | 0 | |a Isolation and identification of Streptomyces sp. Act4Zk, a good producer of Staurosporine and some derivatives |
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| 500 | |a Date Revised 16.03.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 The Society for Applied Microbiology. | ||
| 520 | |a In this study, strain Streptomyces sp. Act4Zk was isolated based on a method developed for the isolation of myxobacteria. Due to the low efficiency of the majority of conventional DNA extraction techniques, for molecular identification of the strain Streptomyces sp. Act4Zk, a new technique for DNA extraction of Actinobacteria was developed. In order to explore potential bioactivities of the strain, extracts of the fermented broth culture were prepared by an organic solvent (i.e. ethyl acetate) extraction method using. These ethyl acetate extracts were subjected to HPLC fractionation against standard micro-organisms, followed by LC/MS analysis. Based on morphological, physiological, biochemical and 16S rRNA gene sequence data, strain Streptomyces sp. Act4Zk is likely to be a new species of Streptomyces, close to Streptomyces genecies and Streptomyces roseolilacinus. Antimicrobial assay indicated high antifungal activity as well as antibacterial activity against Mycobacterium smegmatis and Gram-positive bacteria for the new strain. HPLC and LC/MS analyses of the extracts led to the identification of three different compounds and confirmed our hypothesis that the interesting species of the genus Streptomyces being a good producer of staurosporine and some derivatives | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Streptomyces | |
| 650 | 4 | |a DNA extraction method | |
| 650 | 4 | |a Stausporine | |
| 650 | 4 | |a antibacterial activity | |
| 650 | 4 | |a antifungal activity | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Antifungal Agents |2 NLM | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a RNA, Ribosomal, 16S |2 NLM | |
| 650 | 7 | |a Staurosporine |2 NLM | |
| 650 | 7 | |a H88EPA0A3N |2 NLM | |
| 700 | 1 | |a Ebrahimipour, G |e verfasserin |4 aut | |
| 700 | 1 | |a Wink, J |e verfasserin |4 aut | |
| 700 | 1 | |a Narmani, A |e verfasserin |4 aut | |
| 700 | 1 | |a Risdian, C |e verfasserin |4 aut | |
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