Bioglass could increase cell membrane fluidity with ion products to develop its bioactivity

© 2020 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd..

OBJECTIVES: Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear.

MATERIALS AND METHODS: Human umbilical vein endothelial cells and human bone marrow stromal cells were used in this study. Fluorescence recovery after photobleaching and generalized polarization was used to characterize changes in cell membrane fluidity. Migration, differentiation and apoptosis experiments were carried out. RNA and protein chip were detected. The signal cascade is simulated to evaluate the effect of increased cell membrane fluidity on signal transduction.

RESULTS: We have demonstrated that ion products released from BG could effectively enhance cell membrane fluidity in a direct and physical way, and Si ions may play a major role. Bioactivities of BG ion products on cells, such as migration and differentiation, were regulated by membrane fluidity. Furthermore, we have proved that BG ion products could promote apoptosis of injured cells based on our conclusion that BG ion products increased membrane fluidity.

CONCLUSIONS: This study proved that BG ion products could develop its bioactivity on cells by directly enhancing cell membrane fluidity and subsequently affected cell behaviours, which may provide an explanation for the general bioactivities of silicate material.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:53

Enthalten in:

Cell proliferation - 53(2020), 11 vom: 15. Nov., Seite e12906

Sprache:

Englisch

Beteiligte Personen:

Yan, Longxin [VerfasserIn]
Li, Haiyan [VerfasserIn]
Xia, Weiliang [VerfasserIn]

Links:

Volltext

Themen:

Biocompatible Materials
Bioglass
Cations, Monovalent
Cell activity
Ions
Journal Article
Membrane fluidity
Silicon
Z4152N8IUI

Anmerkungen:

Date Completed 20.11.2020

Date Revised 20.11.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/cpr.12906

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM316133299