Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein

Copyright © 2020 Elsevier Inc. All rights reserved..

Previous study has shown that Hibiscus sulfite oxidase (SO) interacts with Hibiscus chlorotic ringspot virus (HCRSV) coat protein (CP) and triggers sulfur enhanced defense (SED). In this study, we show the interaction of Arabidopsis SO (AtSO) and Turnip crinkle virus (TCV) CP in Arabidopsis thaliana plants. We identified the binding sites of TCV CP (W274) and AtSO (D223) using bioinformatics and confirmed it experimentally. Mutation of binding site W274 to A274 in TCV CP resulted in failure of TCV infection. TCV accumulation in SO over-expression (SO_OE) plants was lower than that in wild-type (WT) and SO knock-out (SO_KO) plants at 7 dpi but reached a level similar to that of WT and SO_KO plants at 10 dpi. AtSO competed with Argonaute 1 (AGO1) for TCV CP binding in vitro. AtSO may serve as an anti-viral factor through sequestering TCV CP for binding with AGO1 and confers virus resistance.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:551

Enthalten in:

Virology - 551(2020) vom: 01. Dez., Seite 64-74

Sprache:

Englisch

Beteiligte Personen:

Wu, Chao [VerfasserIn]
Kannan, Srinivasaraghavan [VerfasserIn]
Verma, Chandra S [VerfasserIn]
Swaminathan, Kunchithapadam [VerfasserIn]
Wong, Sek-Man [VerfasserIn]

Links:

Volltext

Themen:

AGO1 protein, Arabidopsis
Ago1
Arabidopsis Proteins
Arabidopsis sulfite oxidase
Argonaute Proteins
Capsid Proteins
Coat protein
EC 1.8.3.1
Journal Article
Research Support, Non-U.S. Gov't
Sulfite Oxidase
Turnip crinkle virus

Anmerkungen:

Date Completed 22.02.2021

Date Revised 22.02.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.virol.2020.08.013

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM316085898