Direct evidence for crossover and chromatid interference in meiosis of two plant hybrids (Lolium multiflorum×Festuca pratensis and Allium cepa×A. roylei)

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology..

Crossing over, in addition to its strictly genetic role, also performs a critical mechanical function, by bonding homologues in meiosis. Hence, it is responsible for an orderly reduction of the chromosome number. As such, it is strictly controlled in frequency and distribution. The well-known crossover control is positive crossover interference which reduces the probability of a crossover in the vicinity of an already formed crossover. A poorly studied aspect of the control is chromatid interference. Such analyses are possible in very few organisms as they require observation of all four products of a single meiosis. Here, we provide direct evidence of chromatid interference. Using in situ probing in two interspecific plant hybrids (Lolium multiflorum×Festuca pratensis and Allium cepa×A. roylei) during anaphase I, we demonstrate that the involvement of four chromatids in double crossovers is significantly more frequent than expected (64% versus 25%). We also provide a physical measure of the crossover interference distance, covering ~30-40% of the relative chromosome arm length, and show that the centromere acts as a barrier for crossover interference. The two arms of a chromosome appear to act as independent units in the process of crossing over. Chromatid interference has to be seriously addressed in genetic mapping approaches and further studies.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:72

Enthalten in:

Journal of experimental botany - 72(2021), 2 vom: 02. Feb., Seite 254-267

Sprache:

Englisch

Beteiligte Personen:

Ferreira, Marco Tulio Mendes [VerfasserIn]
Glombik, Marek [VerfasserIn]
Perničková, Kateřina [VerfasserIn]
Duchoslav, Martin [VerfasserIn]
Scholten, Olga [VerfasserIn]
Karafiátová, Miroslava [VerfasserIn]
Techio, Vania Helena [VerfasserIn]
Doležel, Jaroslav [VerfasserIn]
Lukaszewski, Adam J [VerfasserIn]
Kopecký, David [VerfasserIn]

Links:

Volltext

Themen:

Centromere
Chromatid interference
Crossover interference
Homoeologous chromosome
Hybrid
Journal Article
Meiosis
Recombination
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Anmerkungen:

Date Completed 13.05.2021

Date Revised 13.05.2021

published: Print

Citation Status MEDLINE

doi:

10.1093/jxb/eraa455

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM315997435