CRISPRoff enables spatio-temporal control of CRISPR editing
Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Nature communications - 11(2020), 1 vom: 07. Okt., Seite 5041 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Carlson-Stevermer, Jared [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 27.10.2020 Date Revised 29.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-020-18853-3 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination | ||
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| 700 | 1 | |a Stoner, Rich |e verfasserin |4 aut | |
| 700 | 1 | |a Maures, Travis |e verfasserin |4 aut | |
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