Fluorinated polymer emulsion systems : Construction and application in delivering genes and proteins
Copyright © 2020 Elsevier Masson SAS. All rights reserved..
Emulsions have shown great potential in the delivery of various types of cargoes such as nucleic acids and proteins. In this study, fluorinated polymer emulsions (PFxPFD-n) were prepared using fluorinated polymers with different structures as surfactant in PFD emulsions under ultrasound. These polymer emulsions gave comparable DNA binding ability compared with corresponding polymers. Heparin competition experiment showed that polymer emulsions could compact DNA or protein to form more stable complexes. In vitro gene transfection results showed that the polymer emulsions could induce higher gene expression than corresponding polymers and polyethyleneimine (PEI), and the transfection efficiency was enhanced with the increase of PFD amount in polymer emulsions. Flow cytometry studies revealed that the emulsions could mediate more efficient cellular uptake with stronger serum tolerance. Moreover, the polymer emulsion could deliver considerable amount of OVA into Raw 264.7 cells at low mass ratio, showing its potential in immunotherapy. The activities of β-galactosidase delivered by the emulsions could also be well maintained after entering cells. This study provides a strategy to construct cationic gene and cytosolic protein vectors with high efficiency and low toxicity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:207 |
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| Enthalten in: |
European journal of medicinal chemistry - 207(2020) vom: 01. Dez., Seite 112799 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiao, Ya-Ping [VerfasserIn] |
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| Links: |
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| Themen: |
9002-98-6 |
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| Anmerkungen: |
Date Completed 20.04.2021 Date Revised 20.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ejmech.2020.112799 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315514590 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Masson SAS. All rights reserved. | ||
| 520 | |a Emulsions have shown great potential in the delivery of various types of cargoes such as nucleic acids and proteins. In this study, fluorinated polymer emulsions (PFxPFD-n) were prepared using fluorinated polymers with different structures as surfactant in PFD emulsions under ultrasound. These polymer emulsions gave comparable DNA binding ability compared with corresponding polymers. Heparin competition experiment showed that polymer emulsions could compact DNA or protein to form more stable complexes. In vitro gene transfection results showed that the polymer emulsions could induce higher gene expression than corresponding polymers and polyethyleneimine (PEI), and the transfection efficiency was enhanced with the increase of PFD amount in polymer emulsions. Flow cytometry studies revealed that the emulsions could mediate more efficient cellular uptake with stronger serum tolerance. Moreover, the polymer emulsion could deliver considerable amount of OVA into Raw 264.7 cells at low mass ratio, showing its potential in immunotherapy. The activities of β-galactosidase delivered by the emulsions could also be well maintained after entering cells. This study provides a strategy to construct cationic gene and cytosolic protein vectors with high efficiency and low toxicity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cationic vectors | |
| 650 | 4 | |a Gene delivery | |
| 650 | 4 | |a Polymer emulsion | |
| 650 | 4 | |a Protein delivery | |
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| 650 | 7 | |a Emulsions |2 NLM | |
| 650 | 7 | |a Polymers |2 NLM | |
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| 700 | 1 | |a Liu, Yan-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xiao-Qi |e verfasserin |4 aut | |
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