Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc
Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2221 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2221(2021) vom: 26., Seite 41-52 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
van den Akker, Guus G H [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Annulus fibrosus |
|---|
| Anmerkungen: |
Date Completed 30.03.2021 Date Revised 30.03.2021 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/978-1-0716-0989-7_4 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM315499214 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM315499214 | ||
| 003 | DE-627 | ||
| 005 | 20231225155040.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-0716-0989-7_4 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1051.xml |
| 035 | |a (DE-627)NLM315499214 | ||
| 035 | |a (NLM)32979197 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a van den Akker, Guus G H |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 30.03.2021 | ||
| 500 | |a Date Revised 30.03.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Annulus fibrosus | |
| 650 | 4 | |a Cell characterization | |
| 650 | 4 | |a Cell isolation | |
| 650 | 4 | |a Collagenase | |
| 650 | 4 | |a Intervertebral disc | |
| 650 | 4 | |a Nucleus pulposus | |
| 700 | 1 | |a Cremers, Andy |e verfasserin |4 aut | |
| 700 | 1 | |a Surtel, Donatus A M |e verfasserin |4 aut | |
| 700 | 1 | |a Voncken, Willem |e verfasserin |4 aut | |
| 700 | 1 | |a Welting, Tim J M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 2221(2021) vom: 26., Seite 41-52 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:2221 |g year:2021 |g day:26 |g pages:41-52 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-0716-0989-7_4 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 2221 |j 2021 |b 26 |h 41-52 | ||