Tacrolimus, a rapamycin target protein inhibitor, exerts anti-cystic echinococcosis effects both in vitro and in vivo

Copyright © 2020 Elsevier B.V. All rights reserved..

In view of a growing need for new treatment options for human cystic echinococcosis (CE), we aimed to investigate the efficacy of mTOR pathway inhibitors against CE in vitro and in vivo. Among the seven mTOR inhibitors evaluated, tacrolimus (TAC) showed significant dose- and time-dependent killing of cultured protoscoleces and cysts in vitro. Notably, the oral administration of TAC (4 mg/kg/day) to CE mice model highly effectively reduced both the weight and number of parasitic cysts. Transmission electron microscopy revealed that TAC destroys the ultrastructure of cysts, both in vitro and in vivo. Furthermore, TAC had no significant effect on blood glucose, body weight, liver, or kidney functions in mice. We further observed that the ATP levels and glucose content of cysts reduced upon TAC treatment, indicating that inhibiting mTORC1 activity possibly affects glucose metabolism in the cysts of mice. Based on our experimental data, TAC emerged as a promising anti-cyst drug that efficiently inhibits the growth of cysts.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:212

Enthalten in:

Acta tropica - 212(2020) vom: 30. Dez., Seite 105708

Sprache:

Englisch

Beteiligte Personen:

Muhedier, Muzhabaier [VerfasserIn]
Li, Jintian [VerfasserIn]
Liu, Hui [VerfasserIn]
Ma, Guizhi [VerfasserIn]
Amahong, Kuerbannisha [VerfasserIn]
Lin, Renyong [VerfasserIn]
Lü, Guodong [VerfasserIn]

Links:

Volltext

Themen:

Anti-cystic echinococcosis
Antiparasitic Agents
Cystic echinococcosis
EC 2.7.11.1
Glucose
IY9XDZ35W2
Journal Article
MTOR inhibitors
TOR Serine-Threonine Kinases
Tacrolimus
WM0HAQ4WNM

Anmerkungen:

Date Completed 05.03.2021

Date Revised 04.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.actatropica.2020.105708

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM315279028