Efficient biosynthesis of polysaccharide welan gum in heat shock protein-overproducing Sphingomonas sp. via temperature-dependent strategy
Cell growth and product formation are two critical processes in polysaccharide welan biosynthesis, but the conflict between them is often encountered. In this study, a temperature-dependent strategy was designed for two-stage welan production through overexpressing heat shock proteins in Sphingomonas sp. The first stage was cell growth phase with higher TCA cycle activity at 42 °C; the second stage was welan formation phase with higher precursor synthesis pathway activity at 37 °C. The highest welan concentration 37.5 g/L was achieved after two-stage process. Ultimately, this strategy accumulated welan yield of 79.2 g/100 g glucose and productivity of 0.62 g/L/h at 60 h, which were the best reported results so far. The duration of fermentation was shortened. Besides, rheological behavior of welan gum solutions remained stable at wide range of temperature, pH, and NaCl. These results indicated that this approach efficiently improved welan synthesis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Bioprocess and biosystems engineering - 44(2021), 2 vom: 17. Feb., Seite 247-257 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhu, Ping [VerfasserIn] |
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| Links: |
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| Themen: |
96949-22-3 |
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| Anmerkungen: |
Date Completed 27.10.2021 Date Revised 27.10.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00449-020-02438-x |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315163453 |
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| 245 | 1 | 0 | |a Efficient biosynthesis of polysaccharide welan gum in heat shock protein-overproducing Sphingomonas sp. via temperature-dependent strategy |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Cell growth and product formation are two critical processes in polysaccharide welan biosynthesis, but the conflict between them is often encountered. In this study, a temperature-dependent strategy was designed for two-stage welan production through overexpressing heat shock proteins in Sphingomonas sp. The first stage was cell growth phase with higher TCA cycle activity at 42 °C; the second stage was welan formation phase with higher precursor synthesis pathway activity at 37 °C. The highest welan concentration 37.5 g/L was achieved after two-stage process. Ultimately, this strategy accumulated welan yield of 79.2 g/100 g glucose and productivity of 0.62 g/L/h at 60 h, which were the best reported results so far. The duration of fermentation was shortened. Besides, rheological behavior of welan gum solutions remained stable at wide range of temperature, pH, and NaCl. These results indicated that this approach efficiently improved welan synthesis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biosynthesis | |
| 650 | 4 | |a Heat shock protein | |
| 650 | 4 | |a Temperature dependent | |
| 650 | 4 | |a Two-stage fermentation | |
| 650 | 4 | |a Welan gum | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Heat-Shock Proteins |2 NLM | |
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| 700 | 1 | |a Zhan, Yijing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Cheng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoliu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Liming |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Hong |e verfasserin |4 aut | |
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