Control of Cell Migration Using Optogenetics
Optogenetics uses light to manipulate protein localization or activity from subcellular to supra-cellular level with unprecedented spatiotemporal resolution. We used it to control the activity of the Cdc42 Rho GTPase, a major regulator of actin polymerization and cell polarity. In this chapter, we describe how to trigger and guide cell migration using optogenetics as a way to mimic EMT in an artificial yet highly controllable fashion.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2179 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2179(2021) vom: 16., Seite 415-425 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Valon, Leo [VerfasserIn] |
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| Links: |
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| Themen: |
CDC42 protein, human |
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| Anmerkungen: |
Date Completed 19.03.2021 Date Revised 19.03.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-0779-4_29 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Optogenetics uses light to manipulate protein localization or activity from subcellular to supra-cellular level with unprecedented spatiotemporal resolution. We used it to control the activity of the Cdc42 Rho GTPase, a major regulator of actin polymerization and cell polarity. In this chapter, we describe how to trigger and guide cell migration using optogenetics as a way to mimic EMT in an artificial yet highly controllable fashion | ||
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