In Vivo Detection of Extracellular Adenosine Triphosphate in a Mouse Model of Traumatic Brain Injury
Traumatic brain injury (TBI) is traditionally characterized by primary and secondary injury phases, both contributing to pathological and morphological changes. The mechanisms of damage and chronic consequences of TBI remain to be fully elucidated, but synaptic homeostasis disturbances and impaired energy metabolism are proposed to be a major contributor. It has been proposed that an increase of extracellular (eATP) adenosine triphosphate (ATP) in the area immediately surrounding impact may play a pivotal role in this sequence of events. After tissue injury, rupture of cell membranes allows release of intracellular ATP into the extracellular space, triggering a cascade of toxic events and inflammation. ATP is a ubiquitous messenger; however, simple and reliable techniques to measure its concentration have proven elusive. Here, we integrate a sensitive bioluminescent eATP sensor known as pmeLUC, with a controlled cortical impact mouse model to monitor eATP changes in a living animal after injury. Using the pmeLUC probe, a rapid increase of eATP is observed proximal to the point of impact within minutes of the injury. This event is significantly attenuated when animals are pretreated with an ATP hydrolyzing agent (apyrase) before surgery, confirming the contribution of eATP. This new eATP reporter could be useful for understanding the role of eATP in the pathogenesis in TBI and may identify a window of opportunity for therapeutic intervention.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
|---|---|
| Enthalten in: |
Journal of neurotrauma - 38(2021), 5 vom: 16. März, Seite 655-664 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Faroqi, Ayman H [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
8L70Q75FXE |
|---|
| Anmerkungen: |
Date Completed 07.01.2022 Date Revised 07.01.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1089/neu.2020.7226 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM315075007 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM315075007 | ||
| 003 | DE-627 | ||
| 005 | 20231225154126.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1089/neu.2020.7226 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1050.xml |
| 035 | |a (DE-627)NLM315075007 | ||
| 035 | |a (NLM)32935624 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Faroqi, Ayman H |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a In Vivo Detection of Extracellular Adenosine Triphosphate in a Mouse Model of Traumatic Brain Injury |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 07.01.2022 | ||
| 500 | |a Date Revised 07.01.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Traumatic brain injury (TBI) is traditionally characterized by primary and secondary injury phases, both contributing to pathological and morphological changes. The mechanisms of damage and chronic consequences of TBI remain to be fully elucidated, but synaptic homeostasis disturbances and impaired energy metabolism are proposed to be a major contributor. It has been proposed that an increase of extracellular (eATP) adenosine triphosphate (ATP) in the area immediately surrounding impact may play a pivotal role in this sequence of events. After tissue injury, rupture of cell membranes allows release of intracellular ATP into the extracellular space, triggering a cascade of toxic events and inflammation. ATP is a ubiquitous messenger; however, simple and reliable techniques to measure its concentration have proven elusive. Here, we integrate a sensitive bioluminescent eATP sensor known as pmeLUC, with a controlled cortical impact mouse model to monitor eATP changes in a living animal after injury. Using the pmeLUC probe, a rapid increase of eATP is observed proximal to the point of impact within minutes of the injury. This event is significantly attenuated when animals are pretreated with an ATP hydrolyzing agent (apyrase) before surgery, confirming the contribution of eATP. This new eATP reporter could be useful for understanding the role of eATP in the pathogenesis in TBI and may identify a window of opportunity for therapeutic intervention | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CCI | |
| 650 | 4 | |a TBI | |
| 650 | 4 | |a extracellular ATP | |
| 650 | 4 | |a in vivo Imaging | |
| 650 | 4 | |a pmeLUC | |
| 650 | 7 | |a Adenosine Triphosphate |2 NLM | |
| 650 | 7 | |a 8L70Q75FXE |2 NLM | |
| 650 | 7 | |a Apyrase |2 NLM | |
| 650 | 7 | |a EC 3.6.1.5 |2 NLM | |
| 700 | 1 | |a Lim, Melina J |e verfasserin |4 aut | |
| 700 | 1 | |a Kee, Emma C |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jannifer H |e verfasserin |4 aut | |
| 700 | 1 | |a Burgess, Jeremy D |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Ridong |e verfasserin |4 aut | |
| 700 | 1 | |a Di Virgilio, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Delenclos, Marion |e verfasserin |4 aut | |
| 700 | 1 | |a McLean, Pamela J |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of neurotrauma |d 1996 |g 38(2021), 5 vom: 16. März, Seite 655-664 |w (DE-627)NLM01272498X |x 1557-9042 |7 nnns |
| 773 | 1 | 8 | |g volume:38 |g year:2021 |g number:5 |g day:16 |g month:03 |g pages:655-664 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1089/neu.2020.7226 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 38 |j 2021 |e 5 |b 16 |c 03 |h 655-664 | ||