Poritiphilus flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coral Porites lutea
A novel Gram-stain-negative, non-endospore-forming, non-motile, aerobic bacterium (strain R33T) was isolated from coral Porites lutea and subjected to a polyphasic taxonomic study. The G+C content was 44.5 mol%. The only detected respiratory quinone was menaquinone 6 (MK-6). The major cellular fatty acids were iso-C15 : 0 and iso-C15 : 1 ω6c. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Global alignment based on 16S rRNA gene sequences indicated that strain R33T shares the highest sequence identity of 93.2 % with Muriicola marianensis A6B8T in the family Flavobacteriaceae. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R33T forms a distinct branch in a stable clade comprising strain R33T and members of the genera Muriicola, Robiginitalea, Eudoraea and Zeaxanthinibacter. The phylogenomic analysis also supported this 16S rRNA gene-based phylogenetic result. Comparative genomic analysis indicated that strain R33T is rich in AraC-type DNA-binding domain-containing protein-coding genes, which means the regulation of carbon utilization is very complex. Low 16S rRNA gene identity, different polar lipids and/or cellular fatty acid profiles could readily distinguish strain R33T from any validly published type strains. Therefore, strain R33T is suggested to represent a new species in a new genus, for which the name Poritiphilus flavus gen. nov., sp. nov. is proposed. The type strain is R33T (=MCCC 1K03853T=KCTC 72443T).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:70 |
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| Enthalten in: |
International journal of systematic and evolutionary microbiology - 70(2020), 11 vom: 14. Nov., Seite 5620-5626 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Guanghua [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.12.2020 Date Revised 29.12.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.1099/ijsem.0.004452 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM314969918 |
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| 100 | 1 | |a Wang, Guanghua |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Poritiphilus flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coral Porites lutea |
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| 500 | |a Date Completed 29.12.2020 | ||
| 500 | |a Date Revised 29.12.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A novel Gram-stain-negative, non-endospore-forming, non-motile, aerobic bacterium (strain R33T) was isolated from coral Porites lutea and subjected to a polyphasic taxonomic study. The G+C content was 44.5 mol%. The only detected respiratory quinone was menaquinone 6 (MK-6). The major cellular fatty acids were iso-C15 : 0 and iso-C15 : 1 ω6c. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Global alignment based on 16S rRNA gene sequences indicated that strain R33T shares the highest sequence identity of 93.2 % with Muriicola marianensis A6B8T in the family Flavobacteriaceae. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R33T forms a distinct branch in a stable clade comprising strain R33T and members of the genera Muriicola, Robiginitalea, Eudoraea and Zeaxanthinibacter. The phylogenomic analysis also supported this 16S rRNA gene-based phylogenetic result. Comparative genomic analysis indicated that strain R33T is rich in AraC-type DNA-binding domain-containing protein-coding genes, which means the regulation of carbon utilization is very complex. Low 16S rRNA gene identity, different polar lipids and/or cellular fatty acid profiles could readily distinguish strain R33T from any validly published type strains. Therefore, strain R33T is suggested to represent a new species in a new genus, for which the name Poritiphilus flavus gen. nov., sp. nov. is proposed. The type strain is R33T (=MCCC 1K03853T=KCTC 72443T) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Porites lutea | |
| 650 | 4 | |a Poritiphilus flavus | |
| 650 | 4 | |a pure culture | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a Fatty Acids |2 NLM | |
| 650 | 7 | |a Phosphatidylethanolamines |2 NLM | |
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| 650 | 7 | |a Vitamin K 2 |2 NLM | |
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| 650 | 7 | |a menaquinone 6 |2 NLM | |
| 650 | 7 | |a 71ANL51TLA |2 NLM | |
| 700 | 1 | |a Xu, Shuailiang |e verfasserin |4 aut | |
| 700 | 1 | |a Dang, Ge |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jianfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Hongfei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Biao |e verfasserin |4 aut | |
| 700 | 1 | |a Liao, Zhiheng |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Jiayuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yinghui |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Kefu |e verfasserin |4 aut | |
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