A Novel Hybrid Silk Fibroin/Polyurethane Arteriovenous Graft for Hemodialysis : Proof-of-Concept Animal Study in an Ovine Model
© 2020 Wiley-VCH GmbH..
To solve the problem of vascular access failure, a novel semi-degradable hybrid vascular graft, manufactured by electrospinning using silk fibroin and polyurethane (Silkothane), has been previously developed and characterized in vitro. This proof-of-concept animal study aims at evaluating the performances of Silkothane grafts in a sheep model of arteriovenous shunt, in terms of patency and short-term remodeling. Nine Silkothane grafts are implanted between the common carotid artery and the external jugular vein of nine sheep, examined by palpation three times per week, by echo-color Doppler every two weeks, and euthanized at 30, 60, and 90 days (N = 3 per group). At sacrifice, grafts are harvested and submitted for histopathology and/or scanning electron microcopy (SEM). No cases of graft-related complications are recorded. Eight of nine sheep (89%) show 100% primary unassisted patency at the respective time of sacrifice (flow rate 1.76 ± 0.61 L min-1 , one case of surgery-related thrombosis excluded). Histopathology and SEM analysis evidence signs of inflammation and pseudointima inside the graft lumen, especially at the venous anastomosis; however, endoluminal stenosis never impairs the functionality of the shunt and coverage by endothelial cells is observed. In this model, Silkothane grafts grant safety and 100% patency up to 90 days.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
Advanced healthcare materials - 9(2020), 20 vom: 09. Okt., Seite e2000794 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Riboldi, Stefania Adele [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
9002-84-0 |
|---|
| Anmerkungen: |
Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/adhm.202000794 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM314867783 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM314867783 | ||
| 003 | DE-627 | ||
| 005 | 20231225153651.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/adhm.202000794 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1049.xml |
| 035 | |a (DE-627)NLM314867783 | ||
| 035 | |a (NLM)32914588 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Riboldi, Stefania Adele |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A Novel Hybrid Silk Fibroin/Polyurethane Arteriovenous Graft for Hemodialysis |b Proof-of-Concept Animal Study in an Ovine Model |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.05.2021 | ||
| 500 | |a Date Revised 14.05.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Wiley-VCH GmbH. | ||
| 520 | |a To solve the problem of vascular access failure, a novel semi-degradable hybrid vascular graft, manufactured by electrospinning using silk fibroin and polyurethane (Silkothane), has been previously developed and characterized in vitro. This proof-of-concept animal study aims at evaluating the performances of Silkothane grafts in a sheep model of arteriovenous shunt, in terms of patency and short-term remodeling. Nine Silkothane grafts are implanted between the common carotid artery and the external jugular vein of nine sheep, examined by palpation three times per week, by echo-color Doppler every two weeks, and euthanized at 30, 60, and 90 days (N = 3 per group). At sacrifice, grafts are harvested and submitted for histopathology and/or scanning electron microcopy (SEM). No cases of graft-related complications are recorded. Eight of nine sheep (89%) show 100% primary unassisted patency at the respective time of sacrifice (flow rate 1.76 ± 0.61 L min-1 , one case of surgery-related thrombosis excluded). Histopathology and SEM analysis evidence signs of inflammation and pseudointima inside the graft lumen, especially at the venous anastomosis; however, endoluminal stenosis never impairs the functionality of the shunt and coverage by endothelial cells is observed. In this model, Silkothane grafts grant safety and 100% patency up to 90 days | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a hemodialysis | |
| 650 | 4 | |a hybrid vascular grafts | |
| 650 | 4 | |a ovine models | |
| 650 | 4 | |a silk fibroin | |
| 650 | 4 | |a vascular access | |
| 650 | 7 | |a Polyurethanes |2 NLM | |
| 650 | 7 | |a Polytetrafluoroethylene |2 NLM | |
| 650 | 7 | |a 9002-84-0 |2 NLM | |
| 650 | 7 | |a Fibroins |2 NLM | |
| 650 | 7 | |a 9007-76-5 |2 NLM | |
| 700 | 1 | |a Tozzi, Matteo |e verfasserin |4 aut | |
| 700 | 1 | |a Bagardi, Mara |e verfasserin |4 aut | |
| 700 | 1 | |a Ravasio, Giuliano |e verfasserin |4 aut | |
| 700 | 1 | |a Cigalino, Giorgio |e verfasserin |4 aut | |
| 700 | 1 | |a Crippa, Luca |e verfasserin |4 aut | |
| 700 | 1 | |a Piccolo, Solange |e verfasserin |4 aut | |
| 700 | 1 | |a Nahal, Amal |e verfasserin |4 aut | |
| 700 | 1 | |a Spandri, Mattia |e verfasserin |4 aut | |
| 700 | 1 | |a Catto, Valentina |e verfasserin |4 aut | |
| 700 | 1 | |a Tironi, Matteo |e verfasserin |4 aut | |
| 700 | 1 | |a Greco, Francesco Giovanni |e verfasserin |4 aut | |
| 700 | 1 | |a Remuzzi, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Acocella, Fabio |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Advanced healthcare materials |d 2012 |g 9(2020), 20 vom: 09. Okt., Seite e2000794 |w (DE-627)NLM218710070 |x 2192-2659 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2020 |g number:20 |g day:09 |g month:10 |g pages:e2000794 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/adhm.202000794 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2020 |e 20 |b 09 |c 10 |h e2000794 | ||