Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis

© 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd..

AIM: To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2 ], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 in periapical tissues.

METHODOLOGY: Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: [Ca(OH)2 ] + saline solution (SSL) [Ca(OH)2  + SSL group] (n = 12), Ca(OH)2  + 2% chlorhexidine gel [Ca(OH)2  + 2% CHX gel group] (n = 12) and NAC [NAC group] (n = 12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications (S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test and Kruskal-Wallis test, followed by Dunn's post hoc test; all statistical tests were performed at a significance level of 5%.

RESULTS: RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH)2 medication did not increase the levels of RvE1 or RvD2 (both P > 0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (P < 0.05).

CONCLUSIONS: RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:54

Enthalten in:

International endodontic journal - 54(2021), 1 vom: 07. Jan., Seite 61-73

Sprache:

Englisch

Beteiligte Personen:

Corazza, B J M [VerfasserIn]
Martinho, F C [VerfasserIn]
Khoury, R D [VerfasserIn]
Toia, C C [VerfasserIn]
Orozco, E I F [VerfasserIn]
Prado, R F [VerfasserIn]
Machado, F P [VerfasserIn]
Valera, M C [VerfasserIn]

Links:

Volltext

Themen:

Acetylcysteine
Calcium Hydroxide
Calcium hydroxide
Chlorhexidine
Intracanal medication
Journal Article
PF5DZW74VN
R4KO0DY52L
Resolvins
Root Canal Irrigants
Root canal
WYQ7N0BPYC

Anmerkungen:

Date Completed 29.12.2020

Date Revised 29.12.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/iej.13403

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM314687033