Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers
Live cell fluorescence imaging is the method of choice to visualize dynamic cellular processes in time and space, such as adhesion to and invasion of polarized epithelial cells by Salmonella enterica sv. Typhimurium. Scanning electron microscopy provides highest resolution of surface structures of infected cells, providing ultrastructure of the apical side of host cells and infecting Salmonella. Combining both methods toward correlative light and scanning electron microscopy (CLSEM) enables new insights in adhesion and invasion mechanisms regarding dynamics over time, and high spatial resolution with precise time lines. To correlate fast live cell imaging of polarized monolayer cells with scanning electron microscopy, we developed a robust method by using gold mesh grids as convenient CLSEM carriers for standard microscopes. By this, we were able to unravel the morphology of the apical structures of monolayers of polarized epithelial cells at distinct time points during Salmonella infection.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2182 |
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Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2182(2021) vom: 07., Seite 103-115 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Kommnick, Carina [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 18.03.2021 Date Revised 18.03.2021 published: Print Citation Status MEDLINE |
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doi: |
10.1007/978-1-0716-0791-6_10 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM314672125 |
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520 | |a Live cell fluorescence imaging is the method of choice to visualize dynamic cellular processes in time and space, such as adhesion to and invasion of polarized epithelial cells by Salmonella enterica sv. Typhimurium. Scanning electron microscopy provides highest resolution of surface structures of infected cells, providing ultrastructure of the apical side of host cells and infecting Salmonella. Combining both methods toward correlative light and scanning electron microscopy (CLSEM) enables new insights in adhesion and invasion mechanisms regarding dynamics over time, and high spatial resolution with precise time lines. To correlate fast live cell imaging of polarized monolayer cells with scanning electron microscopy, we developed a robust method by using gold mesh grids as convenient CLSEM carriers for standard microscopes. By this, we were able to unravel the morphology of the apical structures of monolayers of polarized epithelial cells at distinct time points during Salmonella infection | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Adhesion | |
650 | 4 | |a CLEM | |
650 | 4 | |a CLSEM | |
650 | 4 | |a Correlative light and electron microscopy | |
650 | 4 | |a Correlative light and scanning electron microscopy | |
650 | 4 | |a Invasion | |
650 | 4 | |a LCI | |
650 | 4 | |a Live cell imaging | |
650 | 4 | |a Mesh grid | |
650 | 4 | |a Monolayer | |
650 | 4 | |a Morphometric analysis | |
650 | 4 | |a Polarized epithelial cells | |
650 | 4 | |a SEM | |
650 | 4 | |a Salmonella enterica serovar Typhimurium | |
650 | 4 | |a Scanning electron microscopy | |
650 | 4 | |a Spinning disc confocal microscopy | |
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