Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers

Live cell fluorescence imaging is the method of choice to visualize dynamic cellular processes in time and space, such as adhesion to and invasion of polarized epithelial cells by Salmonella enterica sv. Typhimurium. Scanning electron microscopy provides highest resolution of surface structures of infected cells, providing ultrastructure of the apical side of host cells and infecting Salmonella. Combining both methods toward correlative light and scanning electron microscopy (CLSEM) enables new insights in adhesion and invasion mechanisms regarding dynamics over time, and high spatial resolution with precise time lines. To correlate fast live cell imaging of polarized monolayer cells with scanning electron microscopy, we developed a robust method by using gold mesh grids as convenient CLSEM carriers for standard microscopes. By this, we were able to unravel the morphology of the apical structures of monolayers of polarized epithelial cells at distinct time points during Salmonella infection.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:2182

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 2182(2021) vom: 07., Seite 103-115

Sprache:

Englisch

Beteiligte Personen:

Kommnick, Carina [VerfasserIn]
Hensel, Michael [VerfasserIn]

Links:

Volltext

Themen:

Adhesion
CLEM
CLSEM
Correlative light and electron microscopy
Correlative light and scanning electron microscopy
Invasion
Journal Article
LCI
Live cell imaging
Mesh grid
Monolayer
Morphometric analysis
Polarized epithelial cells
Research Support, Non-U.S. Gov't
SEM
Salmonella enterica serovar Typhimurium
Scanning electron microscopy
Spinning disc confocal microscopy

Anmerkungen:

Date Completed 18.03.2021

Date Revised 18.03.2021

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-0716-0791-6_10

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM314672125