Cav3.2 T-type calcium channels control acute itch in mice
Cav3.2 T-type calcium channels are important mediators of nociceptive signaling, but their roles in the transmission of itch remains poorly understood. Here we report a key involvement of these channels as key modulators of itch/pruritus-related behavior. We compared scratching behavior responses between wild type and Cav3.2 null mice in models of histamine- or chloroquine-induced itch. We also evaluated the effect of the T-type calcium channel blocker DX332 in male and female wild-type mice injected with either histamine or chloroquine. Cav3.2 null mice exhibited decreased scratching responses during both histamine- and chloroquine-induced acute itch. DX332 co-injected with the pruritogens inhibited scratching responses of male and female mice treated with either histamine or chloroquine. Altogether, our data provide strong evidence that Cav3.2 T-type channels exert an important role in modulating histamine-dependent and -independent itch transmission in the primary sensory afferent pathway, and highlight these channels as potential pharmacological targets to treat pruritus.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Molecular brain - 13(2020), 1 vom: 01. Sept., Seite 119 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gadotti, Vinicius M [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.07.2021 Date Revised 07.07.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s13041-020-00663-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM31446316X |
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| 245 | 1 | 0 | |a Cav3.2 T-type calcium channels control acute itch in mice |
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| 500 | |a Date Revised 07.07.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Cav3.2 T-type calcium channels are important mediators of nociceptive signaling, but their roles in the transmission of itch remains poorly understood. Here we report a key involvement of these channels as key modulators of itch/pruritus-related behavior. We compared scratching behavior responses between wild type and Cav3.2 null mice in models of histamine- or chloroquine-induced itch. We also evaluated the effect of the T-type calcium channel blocker DX332 in male and female wild-type mice injected with either histamine or chloroquine. Cav3.2 null mice exhibited decreased scratching responses during both histamine- and chloroquine-induced acute itch. DX332 co-injected with the pruritogens inhibited scratching responses of male and female mice treated with either histamine or chloroquine. Altogether, our data provide strong evidence that Cav3.2 T-type channels exert an important role in modulating histamine-dependent and -independent itch transmission in the primary sensory afferent pathway, and highlight these channels as potential pharmacological targets to treat pruritus | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cav3.2 T-type channel | |
| 650 | 4 | |a Chloroquine | |
| 650 | 4 | |a Histamine | |
| 650 | 4 | |a Itch | |
| 650 | 4 | |a Pruritus | |
| 650 | 7 | |a Cacna1h protein, mouse |2 NLM | |
| 650 | 7 | |a Calcium Channels, T-Type |2 NLM | |
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| 650 | 7 | |a Chloroquine |2 NLM | |
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| 700 | 1 | |a Kreitinger, Joanna M |e verfasserin |4 aut | |
| 700 | 1 | |a Wageling, Nicholas B |e verfasserin |4 aut | |
| 700 | 1 | |a Budke, Dylan |e verfasserin |4 aut | |
| 700 | 1 | |a Diaz, Philippe |e verfasserin |4 aut | |
| 700 | 1 | |a Zamponi, Gerald W |e verfasserin |4 aut | |
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