GFP Fusion to the N-Terminus of MotB Affects the Proton Channel Activity of the Bacterial Flagellar Motor in Salmonella
The bacterial flagellar motor converts the energy of proton flow through the MotA/MotB complex into mechanical works required for motor rotation. The rotational force is generated by electrostatic interactions between the stator protein MotA and the rotor protein FliG. The Arg-90 and Glu-98 from MotA interact with Asp-289 and Arg-281 of FliG, respectively. An increase in the expression level of the wild-type MotA/MotB complex inhibits motility of the gfp-motBfliG(R281V) mutant but not the fliG(R281V) mutant, suggesting that the MotA/GFP-MotB complex cannot work together with wild-type MotA/MotB in the presence of the fliG(R281V) mutation. However, it remains unknown why. Here, we investigated the effect of the GFP fusion to MotB at its N-terminus on the MotA/MotB function. Over-expression of wild-type MotA/MotB significantly reduced the growth rate of the gfp-motBfliG(R281V) mutant. The over-expression of the MotA/GFP-MotB complex caused an excessive proton leakage through its proton channel, thereby inhibiting cell growth. These results suggest that the GFP tag on the MotB N-terminus affects well-regulated proton translocation through the MotA/MotB proton channel. Therefore, we propose that the N-terminal cytoplasmic tail of MotB couples the gating of the proton channel with the MotA-FliG interaction responsible for torque generation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Biomolecules - 10(2020), 9 vom: 29. Aug. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Morimoto, Yusuke V [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.09.2021 Date Revised 29.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/biom10091255 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM314454101 |
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| 041 | |a eng | ||
| 100 | 1 | |a Morimoto, Yusuke V |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a GFP Fusion to the N-Terminus of MotB Affects the Proton Channel Activity of the Bacterial Flagellar Motor in Salmonella |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 07.09.2021 | ||
| 500 | |a Date Revised 29.03.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The bacterial flagellar motor converts the energy of proton flow through the MotA/MotB complex into mechanical works required for motor rotation. The rotational force is generated by electrostatic interactions between the stator protein MotA and the rotor protein FliG. The Arg-90 and Glu-98 from MotA interact with Asp-289 and Arg-281 of FliG, respectively. An increase in the expression level of the wild-type MotA/MotB complex inhibits motility of the gfp-motBfliG(R281V) mutant but not the fliG(R281V) mutant, suggesting that the MotA/GFP-MotB complex cannot work together with wild-type MotA/MotB in the presence of the fliG(R281V) mutation. However, it remains unknown why. Here, we investigated the effect of the GFP fusion to MotB at its N-terminus on the MotA/MotB function. Over-expression of wild-type MotA/MotB significantly reduced the growth rate of the gfp-motBfliG(R281V) mutant. The over-expression of the MotA/GFP-MotB complex caused an excessive proton leakage through its proton channel, thereby inhibiting cell growth. These results suggest that the GFP tag on the MotB N-terminus affects well-regulated proton translocation through the MotA/MotB proton channel. Therefore, we propose that the N-terminal cytoplasmic tail of MotB couples the gating of the proton channel with the MotA-FliG interaction responsible for torque generation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a bacterial flagellar motor | |
| 650 | 4 | |a fluorescent protein | |
| 650 | 4 | |a ion channel | |
| 650 | 4 | |a proton motive force | |
| 650 | 4 | |a torque generation | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Flig protein, Bacteria |2 NLM | |
| 650 | 7 | |a Molecular Motor Proteins |2 NLM | |
| 650 | 7 | |a MotA protein, Bacteria |2 NLM | |
| 650 | 7 | |a MotB protein, Bacteria |2 NLM | |
| 650 | 7 | |a Protons |2 NLM | |
| 650 | 7 | |a Recombinant Fusion Proteins |2 NLM | |
| 650 | 7 | |a Green Fluorescent Proteins |2 NLM | |
| 650 | 7 | |a 147336-22-9 |2 NLM | |
| 700 | 1 | |a Namba, Keiichi |e verfasserin |4 aut | |
| 700 | 1 | |a Minamino, Tohru |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:10 |g year:2020 |g number:9 |g day:29 |g month:08 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/biom10091255 |3 Volltext |
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