Amyloid-β regulates gap junction protein connexin 43 trafficking in cultured primary astrocytes
© 2020 Maulik et al..
Altered expression and function of astroglial gap junction protein connexin 43 (Cx43) has increasingly been associated to neurotoxicity in Alzheimer disease (AD). Although earlier studies have examined the effect of increased β-amyloid (Aβ) on Cx43 expression and function leading to neuronal damage, underlying mechanisms by which Aβ modulates Cx43 in astrocytes remain elusive. Here, using mouse primary astrocyte cultures, we have examined the cellular processes by which Aβ can alter Cx43 gap junctions. We show that Aβ25-35 impairs functional gap junction coupling yet increases hemichannel activity. Interestingly, Aβ25-35 increased the intracellular pool of Cx43 with a parallel decrease in gap junction assembly at the surface. Intracellular Cx43 was found to be partly retained in the endoplasmic reticulum-associated cell compartments. However, forward trafficking of the newly synthesized Cx43 that already reached the Golgi was not affected in Aβ25-35-exposed astrocytes. Supporting this, treatment with 4-phenylbutyrate, a well-known chemical chaperone that improves trafficking of several transmembrane proteins, restored Aβ-induced impaired gap junction coupling between astrocytes. We further show that interruption of Cx43 endocytosis in Aβ25-35-exposed astrocytes resulted in their retention at the cell surface in the form of functional gap junctions indicating that Aβ25-35 causes rapid internalization of Cx43 gap junctions. Additionally, in silico molecular docking suggests that Aβ can bind favorably to Cx43. Our study thus provides novel insights into the cellular mechanisms by which Aβ modulates Cx43 function in astrocytes, the basic understanding of which is vital for the development of alternative therapeutic strategy targeting connexin channels in AD.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:295 |
|---|---|
| Enthalten in: |
The Journal of biological chemistry - 295(2020), 44 vom: 30. Okt., Seite 15097-15111 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Maulik, Mahua [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 29.03.2021 Date Revised 31.10.2021 published: Print-Electronic PDB: 6MHQ, 2LL2, 1R5S Citation Status MEDLINE |
|---|
| doi: |
10.1074/jbc.RA120.013705 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM31441553X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM31441553X | ||
| 003 | DE-627 | ||
| 005 | 20231225152703.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1074/jbc.RA120.013705 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1048.xml |
| 035 | |a (DE-627)NLM31441553X | ||
| 035 | |a (NLM)32868453 | ||
| 035 | |a (PII)S0021-9258(17)48914-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Maulik, Mahua |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Amyloid-β regulates gap junction protein connexin 43 trafficking in cultured primary astrocytes |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.03.2021 | ||
| 500 | |a Date Revised 31.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a PDB: 6MHQ, 2LL2, 1R5S | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Maulik et al. | ||
| 520 | |a Altered expression and function of astroglial gap junction protein connexin 43 (Cx43) has increasingly been associated to neurotoxicity in Alzheimer disease (AD). Although earlier studies have examined the effect of increased β-amyloid (Aβ) on Cx43 expression and function leading to neuronal damage, underlying mechanisms by which Aβ modulates Cx43 in astrocytes remain elusive. Here, using mouse primary astrocyte cultures, we have examined the cellular processes by which Aβ can alter Cx43 gap junctions. We show that Aβ25-35 impairs functional gap junction coupling yet increases hemichannel activity. Interestingly, Aβ25-35 increased the intracellular pool of Cx43 with a parallel decrease in gap junction assembly at the surface. Intracellular Cx43 was found to be partly retained in the endoplasmic reticulum-associated cell compartments. However, forward trafficking of the newly synthesized Cx43 that already reached the Golgi was not affected in Aβ25-35-exposed astrocytes. Supporting this, treatment with 4-phenylbutyrate, a well-known chemical chaperone that improves trafficking of several transmembrane proteins, restored Aβ-induced impaired gap junction coupling between astrocytes. We further show that interruption of Cx43 endocytosis in Aβ25-35-exposed astrocytes resulted in their retention at the cell surface in the form of functional gap junctions indicating that Aβ25-35 causes rapid internalization of Cx43 gap junctions. Additionally, in silico molecular docking suggests that Aβ can bind favorably to Cx43. Our study thus provides novel insights into the cellular mechanisms by which Aβ modulates Cx43 function in astrocytes, the basic understanding of which is vital for the development of alternative therapeutic strategy targeting connexin channels in AD | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Alzheimer disease | |
| 650 | 4 | |a amyloid-beta (Aβ) | |
| 650 | 4 | |a astrocyte | |
| 650 | 4 | |a connexin 43 (Cx43) | |
| 650 | 4 | |a gap junction | |
| 650 | 4 | |a intracellular trafficking | |
| 650 | 7 | |a Amyloid beta-Peptides |2 NLM | |
| 650 | 7 | |a Connexin 43 |2 NLM | |
| 650 | 7 | |a Phenylbutyrates |2 NLM | |
| 650 | 7 | |a 4-phenylbutyric acid |2 NLM | |
| 650 | 7 | |a 7WY7YBI87E |2 NLM | |
| 700 | 1 | |a Vasan, Lakshmy |e verfasserin |4 aut | |
| 700 | 1 | |a Bose, Abhishek |e verfasserin |4 aut | |
| 700 | 1 | |a Dutta Chowdhury, Saikat |e verfasserin |4 aut | |
| 700 | 1 | |a Sengupta, Neelanjana |e verfasserin |4 aut | |
| 700 | 1 | |a Das Sarma, Jayasri |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The Journal of biological chemistry |d 1945 |g 295(2020), 44 vom: 30. Okt., Seite 15097-15111 |w (DE-627)NLM000004995 |x 1083-351X |7 nnns |
| 773 | 1 | 8 | |g volume:295 |g year:2020 |g number:44 |g day:30 |g month:10 |g pages:15097-15111 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1074/jbc.RA120.013705 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 295 |j 2020 |e 44 |b 30 |c 10 |h 15097-15111 | ||