RPRD1B is a potentially molecular target for diagnosis and prevention of human papillomavirus E6/E7 infection-induced cervical cancer : A case-control study

© 2020 John Wiley & Sons Australia, Ltd..

BACKGROUND: The objective of the study is to investigate the biomarkers for diagnosis and prevention of human papillomavirus (HPV) infection-induced cervical cancer.

METHODS: Cervical cancer tissues were collected from patients with cervical cancer, while noncancer tissues were collected from patients diagnosed with cervical lesions or uterine fibroids at the Chinese PLA General Hospital 301 and 309, China from December 2017 to June 2018. The cancer tissues were collected from the site of lesion, while the noncancer tissues were collected from similar anatomical locations. Quantitative real-time PCR, Western blot (WB), and immunohistochemistry (IHC) were used to detect the mRNA and protein levels of HPV E6/E7, RPRD1B (regulation of nuclear pre-mRNA domain containing 1B), cyclin D1, and transcription factor 4 (TCF4) between cervical cancer tissues and noncancer tissues. The correlation of HPV E6/E7, RPRD1B, cyclin D1, and TCF4 expressions was analyzed.

RESULTS: Twenty patients with cervical cancer and 27 controls without cervical cancer were included in this study. The mRNA expression of HPV E6/E7and RPRD1B was significantly higher in patients with cervical cancer than controls, while cyclin D1 mRNA expression was significantly lower in patients with cervical carcinoma in situ stage, compared with controls. RPRD1B protein expression was significantly higher in patients compared to controls when analyzed by IHC. TCF4 was significantly lower in clinical stage I and Ib of cervical cancer when analyzed by WB. The mRNA and protein expressions of RPRD1B and cyclin D1 were significantly different between patients younger than 50 years old, compared to patients 50 years and older.

CONCLUSIONS: HPV E6/E7 expression was associated with RPRD1B level in cervical cancer. The expression of RPRD1B and cyclin D1 in patients with cervical cancer might be affected by age.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:17

Enthalten in:

Asia-Pacific journal of clinical oncology - 17(2021), 3 vom: 07. Juni, Seite 230-237

Sprache:

Englisch

Beteiligte Personen:

Wen, Na [VerfasserIn]
Bian, Lihua [VerfasserIn]
Gon, Jing [VerfasserIn]
Meng, Yuanguang [VerfasserIn]

Links:

Volltext

Themen:

136601-57-5
CCND1 protein, human
Cell Cycle Proteins
Cervical cancer
Cyclin D1
E6 protein, Human papillomavirus type 16
HPV E6/E7
Journal Article
Neoplasm Proteins
Oncogene Proteins, Viral
Papillomavirus E7 Proteins
QPCR
RNA, Messenger
RPRD1B
RPRD1B protein, human
Repressor Proteins
TCF4 protein, human
Transcription Factor 4
WB

Anmerkungen:

Date Completed 28.06.2021

Date Revised 28.06.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/ajco.13439

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM314394508