The effects of TPL-PEI-CyD on suppressing performance of MCF-7 stem cells

Triptolide, an ingredient of Tripterygium wilfordii, has been demonstrated to possess many biological activities such as immunomodulatory, antitumor activity in experiment. The purpose of this study was to survey the toxicity of TPL-PEI-CyD on renal cells and its effects on breast carcinoma stem cells. The cytotoxicity of TPL-PEI-CyD and TPL on HK-2 was comparatively assessed by CCK-8. After incubation and culturing with TGF-β1, the MCF-7 cells were assessed by flow cytometry for the proportion of CD44+> CD24- cells; then the CD44>+> CD24- cells were sorted by immunomagnetic beads as MCF-7 stem cells. To assess the effect of TPL-PEI-CyD on MCF-7 stem cells, Western Blot was used to detect the expression of Oct-4 and ALDHl in MCF-7 stem cells after being dosed with TPL- PEI-CyD. Results showed that, compared with TPL, the toxicity of TPL-PEI-CyD on HK-2 cells was significantly reduced (P<0.05). Breast carcinoma stem cells can be enriched by TGF-β1 and isolated from MCF-7 cells by immunomagnetic sorting. TPL- PEI-CyD can even more significantly suppress the expression of Oct-4 and ALDHA1 in MCF-7 stem cells than TPL (P<0.05). In conclusion, after coupling TPL and PEI-CyD, TPL-PEI-CyD showed characteristics of effective suppression to breast carcinoma stem cell and decrease of cytotoxicity. It presented the unique effect of traditional Chinese medicine as an efficient and low toxic drug carrier complex for breast carcinoma treatment.

Medienart:

Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:33

Enthalten in:

Pakistan journal of pharmaceutical sciences - 33(2020), 2(Supplementary) vom: 20. März, Seite 835-838

Sprache:

Englisch

Beteiligte Personen:

Zhu, Yimin [VerfasserIn]
Xu, Fuying [VerfasserIn]

Themen:

19ALD1S53J
CD24 Antigen
Cyclodextrins
Diterpenes
Epoxy Compounds
Hyaluronan Receptors
Imines
Journal Article
Phenanthrenes
Poly(ethylene imine)
Polyethylenes
Transforming Growth Factor beta1
Triptolide

Anmerkungen:

Date Completed 22.06.2021

Date Revised 22.06.2021

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM314363815