Clusters of polymorphic transmembrane genes control resistance to schistosomes in snail vectors
© 2020, Tennessen et al..
Schistosomiasis is a debilitating parasitic disease infecting hundreds of millions of people. Schistosomes use aquatic snails as intermediate hosts. A promising avenue for disease control involves leveraging innate host mechanisms to reduce snail vectorial capacity. In a genome-wide association study of Biomphalaria glabrata snails, we identify genomic region PTC2 which exhibits the largest known correlation with susceptibility to parasite infection (>15 fold effect). Using new genome assemblies with substantially higher contiguity than the Biomphalaria reference genome, we show that PTC2 haplotypes are exceptionally divergent in structure and sequence. This variation includes multi-kilobase indels containing entire genes, and orthologs for which most amino acid residues are polymorphic. RNA-Seq annotation reveals that most of these genes encode single-pass transmembrane proteins, as seen in another resistance region in the same species. Such groups of hyperdiverse snail proteins may mediate host-parasite interaction at the cell surface, offering promising targets for blocking the transmission of schistosomiasis.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
eLife - 9(2020) vom: 26. Aug. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Tennessen, Jacob A [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Biomphalaria glabrata |
|---|
| Anmerkungen: |
Date Completed 17.03.2021 Date Revised 17.03.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.7554/eLife.59395 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM314185526 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM314185526 | ||
| 003 | DE-627 | ||
| 005 | 20231225152206.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.7554/eLife.59395 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1047.xml |
| 035 | |a (DE-627)NLM314185526 | ||
| 035 | |a (NLM)32845238 | ||
| 035 | |a (PII)e59395 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tennessen, Jacob A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Clusters of polymorphic transmembrane genes control resistance to schistosomes in snail vectors |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 17.03.2021 | ||
| 500 | |a Date Revised 17.03.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020, Tennessen et al. | ||
| 520 | |a Schistosomiasis is a debilitating parasitic disease infecting hundreds of millions of people. Schistosomes use aquatic snails as intermediate hosts. A promising avenue for disease control involves leveraging innate host mechanisms to reduce snail vectorial capacity. In a genome-wide association study of Biomphalaria glabrata snails, we identify genomic region PTC2 which exhibits the largest known correlation with susceptibility to parasite infection (>15 fold effect). Using new genome assemblies with substantially higher contiguity than the Biomphalaria reference genome, we show that PTC2 haplotypes are exceptionally divergent in structure and sequence. This variation includes multi-kilobase indels containing entire genes, and orthologs for which most amino acid residues are polymorphic. RNA-Seq annotation reveals that most of these genes encode single-pass transmembrane proteins, as seen in another resistance region in the same species. Such groups of hyperdiverse snail proteins may mediate host-parasite interaction at the cell surface, offering promising targets for blocking the transmission of schistosomiasis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a GWAS | |
| 650 | 4 | |a biomphalaria glabrata | |
| 650 | 4 | |a epidemiology | |
| 650 | 4 | |a genetics | |
| 650 | 4 | |a genomics | |
| 650 | 4 | |a global health | |
| 650 | 4 | |a host-parasite | |
| 650 | 4 | |a schistosoma mansoni | |
| 650 | 4 | |a schistosomiasis | |
| 650 | 4 | |a snail | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 700 | 1 | |a Bollmann, Stephanie R |e verfasserin |4 aut | |
| 700 | 1 | |a Peremyslova, Ekaterina |e verfasserin |4 aut | |
| 700 | 1 | |a Kronmiller, Brent A |e verfasserin |4 aut | |
| 700 | 1 | |a Sergi, Clint |e verfasserin |4 aut | |
| 700 | 1 | |a Hamali, Bulut |e verfasserin |4 aut | |
| 700 | 1 | |a Blouin, Michael Scott |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t eLife |d 2012 |g 9(2020) vom: 26. Aug. |w (DE-627)NLM221831460 |x 2050-084X |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2020 |g day:26 |g month:08 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.7554/eLife.59395 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2020 |b 26 |c 08 | ||