Adipocyte Plasma Membrane Protein (APMAP) promotes JC Virus (JCPyV) infection in human glial cells
Copyright © 2020 Elsevier Inc. All rights reserved..
The demyelinating disease progressive multifocal leukoencephalopathy (PML) is caused by the human polyomavirus, JCPyV, under conditions of prolonged immunosuppression. Initial infection is asymptomatic, and the virus establishes lifelong persistence in the host. Following the loss of immune surveillance, the virus can traffic to the central nervous system and infect oligodendrocytes to cause demyelination and PML. The mechanisms involved in glial cell infection are not completely understood. In a screen for N-glycosylated proteins that influence JCPyV pathology, we identified Adipocyte Plasma Membrane Associated Protein (APMAP) as a host cell modulator of JCPyV infection. The removal of APMAP by small interfering siRNA as well as by CRISPR-Cas9 gene editing resulted in a significant decrease in JCPyV infection. Exogenous expression of APMAP in APMAP knockout cell lines rescued susceptibility to infection. These data suggest that virus infection of glial cells is dependent on APMAP.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:548 |
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Enthalten in: |
Virology - 548(2020) vom: 15. Sept., Seite 17-24 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Haley, Sheila A [VerfasserIn] |
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Links: |
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Themen: |
APMAP |
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Anmerkungen: |
Date Completed 15.10.2020 Date Revised 03.09.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.virol.2020.06.002 |
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funding: |
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PPN (Katalog-ID): |
NLM314123776 |
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520 | |a The demyelinating disease progressive multifocal leukoencephalopathy (PML) is caused by the human polyomavirus, JCPyV, under conditions of prolonged immunosuppression. Initial infection is asymptomatic, and the virus establishes lifelong persistence in the host. Following the loss of immune surveillance, the virus can traffic to the central nervous system and infect oligodendrocytes to cause demyelination and PML. The mechanisms involved in glial cell infection are not completely understood. In a screen for N-glycosylated proteins that influence JCPyV pathology, we identified Adipocyte Plasma Membrane Associated Protein (APMAP) as a host cell modulator of JCPyV infection. The removal of APMAP by small interfering siRNA as well as by CRISPR-Cas9 gene editing resulted in a significant decrease in JCPyV infection. Exogenous expression of APMAP in APMAP knockout cell lines rescued susceptibility to infection. These data suggest that virus infection of glial cells is dependent on APMAP | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a APMAP | |
650 | 4 | |a CRISPR | |
650 | 4 | |a Glial cell infection | |
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650 | 4 | |a JCPyV | |
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650 | 7 | |a Membrane Glycoproteins |2 NLM | |
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700 | 1 | |a Atwood, Walter J |e verfasserin |4 aut | |
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