Complete genome of a novel recombinant human astrovirus and its quasispecies in patients following hematopoietic stem cell transplantation

Copyright © 2020 Elsevier B.V. All rights reserved..

Human astroviruses (HAstVs) were first identified in 1975 and can be classified into three clades: classic HAstVs (HAstV 1-8), MLB (MLB1-3) and VA (VA1-5), with MLB and VA were newly identified. Recombination and a high mutation rate make HAstV as one of the rapidly evolving infectious agents. This study reported a novel identified recombinant human astrovirus (Y/1-CHN) and its long existence in two immunocompromised patients with diarrhea following allogeneic hematopoietic stem cell transplantation (allo-HSCT). The identified Yu/1-CHN genome contains 6801 base pairs encoding three open reading frames, with ORF1a best hit to the HAstV1 (Pune strain, 97 % nucleotide identity), while ORF1b and ORF2 best hit to HAstV-5 (DL30 strain, 99 % nucleotide identity). Possible recombination breakpoint was predicted to be located in the boundary of ORF1a and ORF1b. Different quasispecies were found in the host, and the dN/dS ratios of the S and P domains were determined to be 1.189 and 1.444, respectively, suggesting a positive selection existed. Fecal samples collected in different clinical phases from the two patients were all positive for Yu/1-CHN, suggesting a long existence of the virus in the host. It was indicated that immunocompromised patients may a reservoir for astrovirus, their excreta should be monitored even after discharge from hospital.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:288

Enthalten in:

Virus research - 288(2020) vom: 15. Okt., Seite 198138

Sprache:

Englisch

Beteiligte Personen:

Yu, Jie-Mei [VerfasserIn]
Wang, Zhen-Hua [VerfasserIn]
Liu, Na [VerfasserIn]
Zhang, Qing [VerfasserIn]
Dong, Yu-Jun [VerfasserIn]
Duan, Zhao-Jun [VerfasserIn]

Links:

Volltext

Themen:

Allogeneic hematopoietic stem cell transplantation
Human astrovirus
Immunocompromised patient
Journal Article
Recombination
Research Support, Non-U.S. Gov't
Viral quasispecies

Anmerkungen:

Date Completed 09.09.2021

Date Revised 09.09.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.virusres.2020.198138

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM314012656