Synthesis of Graphene Oxide Using Atmospheric Plasma for Prospective Biological Applications

© 2020 Alam et al..

INTRODUCTION: This paper presents a novel technique for the synthesis of graphene oxide (GO) with various surface features using high-density atmospheric plasma deposition. Furthermore, to investigate the use of hydrophobic, super-hydrophobic, and hydrophilic graphene in biological applications, we synthesized hydrophobic, super-hydrophobic, and hydrophilic graphene oxides by additional heat treatment and argon plasma treatment, respectively. In contrast to conventional fabrication procedures, reduced graphene oxide (rGO) formed under low pressure and high-temperature environment using a new synthesis method-developed and described in this study-offers a convenient deposition method on any kind surface with controlled wettability.

METHODS: High density at atmospheric plasma is used for the synthesis of rGO and GO and its biocompatibility based on various wetting properties was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the viability of cells in response to rGO and GO with various surface features was investigated. Structural integrity was characterized by Raman spectroscopy, FESEM and FE-TEM. Wettability was measured via contact angle method and confirmed with XPS analysis.

RESULTS: We found that GO coating with a hydrophilic feature is more biocompatible than other surfaces as observed in case of fibroblast cells. We have shown that wettability-controlled by GO deposition-influences biocompatibilities and antibacterial effect of biomaterial surfaces.

DISCUSSION: Measuring the contact angle, it is found that contact angle for hydrophobic is increased to 150.590 and reduced to 11.580 by heat and argon plasma treatment, respectively, from 75.880 that was initially in the case of hydrophobic surface. XPS analysis confirmed various oxygen-containing functional groups transforming as deposited hydrophobic surface into superhydrophobic and hydrophilic surface. Thus, we have proposed a new, direct, cost-effective, and highly productive method for the synthesis of rGO and GO-with various surface properties-for biological applications. Similarly, for the dental implant application, the Streptococcus mutans was used as an antibacterial effect and found that S. mutans grows slowly on hydrophilic surface. Thus, antibacterial effect was prominent on GO with hydrophilic surface.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

International journal of nanomedicine - 15(2020) vom: 28., Seite 5813-5824

Sprache:

Englisch

Beteiligte Personen:

Alam, Khurshed [VerfasserIn]
Jo, Youn Yi [VerfasserIn]
Park, Chul-Kyu [VerfasserIn]
Cho, Hoonsung [VerfasserIn]

Links:

Volltext

Themen:

059QF0KO0R
7782-42-5
Atmospheric plasma
Biocompatibility
Graphene oxide
Graphite
Hydrophobicity
Journal Article
MTT assay
Plasma Gases
Reduced graphene oxide
Super-hydrophobicity and hydrophilicity
Water

Anmerkungen:

Date Completed 03.09.2020

Date Revised 16.04.2022

published: Electronic-eCollection

Citation Status MEDLINE

doi:

10.2147/IJN.S254860

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM313948291