Use of Ice Recrystallization Inhibition Assays to Screen for Compounds That Inhibit Ice Recrystallization
Ice recrystallization inhibition assays are used to screen for compounds that possess the ability to inhibit ice recrystallization. The most common of these assays are the splat cooling assay (SCA) and sucrose sandwich assay (SSA). These two assays possess similarities; however, they vary in their sample size, cooling rate, and the solution used to dissolve the analyte. In this chapter, both assay methods are described in detail, and we perform a direct comparison of the assays by evaluating the IRI activity of an antifreeze protein (AFP I). IRI activity is quantified by using ImageJ software to analyze ice crystals, and a quantitative value describing the efficiency of the inhibitor is generated. This analysis emphasizes the importance of choosing the right assay to measure IRI activity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2180 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2180(2021) vom: 14., Seite 271-283 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ampaw, Anna A [VerfasserIn] |
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| Links: |
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| Themen: |
Antifreeze Proteins |
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| Anmerkungen: |
Date Completed 15.03.2021 Date Revised 15.03.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-0783-1_9 |
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| funding: |
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NLM313720452 |
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| 520 | |a Ice recrystallization inhibition assays are used to screen for compounds that possess the ability to inhibit ice recrystallization. The most common of these assays are the splat cooling assay (SCA) and sucrose sandwich assay (SSA). These two assays possess similarities; however, they vary in their sample size, cooling rate, and the solution used to dissolve the analyte. In this chapter, both assay methods are described in detail, and we perform a direct comparison of the assays by evaluating the IRI activity of an antifreeze protein (AFP I). IRI activity is quantified by using ImageJ software to analyze ice crystals, and a quantitative value describing the efficiency of the inhibitor is generated. This analysis emphasizes the importance of choosing the right assay to measure IRI activity | ||
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| 700 | 1 | |a Ben, Robert N |e verfasserin |4 aut | |
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