False-positive SARS-CoV-2 serology in 3 children with Kawasaki disease
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved..
BACKGROUND: Kawasaki disease (KD) is an acute febrile and eruptive disease with systemic vasculitis predominantly affecting young East Asian children. Recent reports showed that children with KD-like disease from KD low prevalence regions had positive SARS-CoV-2 serology despite a negative SARS-CoV-2 polymerase chain reaction (PCR) in respiratory samples.
OBJECTIVES: To describe 3 pediatric Kawasaki Disease patients with false positive SARS-CoV-2 serology.
STUDY DESIGN: We retrospectively recruited children with KD diagnosed during the COVID-19 outbreak in Hong Kong. Clinical characteristics and laboratory test results including SARS-CoV-2 PCR results were retrieved. We performed a microparticle-based immunoassay for the detection of IgG against nucleoprotein (NP) and spike protein receptor binding domain (RBD), and a microneutralization assay for the detection of neutralizing antibodies.
RESULTS: Three Chinese children with typical KD were identified. They had no epidemiological links with COVID-19 patients and tested negative for SARS-CoV-2 NPA PCR. They were treated with IVIG and aspirin, and were discharged without complications. Subsequently 2 of them were tested positive against anti-RBD and anti-NP antibodies and 1 was tested positive against anti- RBD antibodies. However, microneutralization assay showed that neutralizing antibodies were absent, suggesting a false-positive IgG result.
CONCLUSION: Detection of neutralizing antibodies is recommended to confirm previous SARS-CoV-2 infection in IgG-positive but PCR-negative patients.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:98 |
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| Enthalten in: |
Diagnostic microbiology and infectious disease - 98(2020), 3 vom: 25. Nov., Seite 115141 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
To, Kelvin Kw [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.10.2020 Date Revised 11.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.diagmicrobio.2020.115141 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM313704376 |
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| 100 | 1 | |a To, Kelvin Kw |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a False-positive SARS-CoV-2 serology in 3 children with Kawasaki disease |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.10.2020 | ||
| 500 | |a Date Revised 11.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: Kawasaki disease (KD) is an acute febrile and eruptive disease with systemic vasculitis predominantly affecting young East Asian children. Recent reports showed that children with KD-like disease from KD low prevalence regions had positive SARS-CoV-2 serology despite a negative SARS-CoV-2 polymerase chain reaction (PCR) in respiratory samples | ||
| 520 | |a OBJECTIVES: To describe 3 pediatric Kawasaki Disease patients with false positive SARS-CoV-2 serology | ||
| 520 | |a STUDY DESIGN: We retrospectively recruited children with KD diagnosed during the COVID-19 outbreak in Hong Kong. Clinical characteristics and laboratory test results including SARS-CoV-2 PCR results were retrieved. We performed a microparticle-based immunoassay for the detection of IgG against nucleoprotein (NP) and spike protein receptor binding domain (RBD), and a microneutralization assay for the detection of neutralizing antibodies | ||
| 520 | |a RESULTS: Three Chinese children with typical KD were identified. They had no epidemiological links with COVID-19 patients and tested negative for SARS-CoV-2 NPA PCR. They were treated with IVIG and aspirin, and were discharged without complications. Subsequently 2 of them were tested positive against anti-RBD and anti-NP antibodies and 1 was tested positive against anti- RBD antibodies. However, microneutralization assay showed that neutralizing antibodies were absent, suggesting a false-positive IgG result | ||
| 520 | |a CONCLUSION: Detection of neutralizing antibodies is recommended to confirm previous SARS-CoV-2 infection in IgG-positive but PCR-negative patients | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a Chinese | |
| 650 | 4 | |a False positive | |
| 650 | 4 | |a Kawasaki disease | |
| 650 | 4 | |a Serology | |
| 650 | 7 | |a Antibodies, Neutralizing |2 NLM | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Coronavirus Nucleocapsid Proteins |2 NLM | |
| 650 | 7 | |a Nucleocapsid Proteins |2 NLM | |
| 650 | 7 | |a Phosphoproteins |2 NLM | |
| 650 | 7 | |a Spike Glycoprotein, Coronavirus |2 NLM | |
| 650 | 7 | |a nucleocapsid phosphoprotein, SARS-CoV-2 |2 NLM | |
| 650 | 7 | |a spike protein, SARS-CoV-2 |2 NLM | |
| 700 | 1 | |a Chua, Gilbert T |e verfasserin |4 aut | |
| 700 | 1 | |a Kwok, Ka Li |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Joshua Sc |e verfasserin |4 aut | |
| 700 | 1 | |a Au, Dennis Chi Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Lam, Yuen Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Wilfred Hs |e verfasserin |4 aut | |
| 700 | 1 | |a Ho, Marco Hk |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Godfrey Cf |e verfasserin |4 aut | |
| 700 | 1 | |a Chui, Celine Sl |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xue |e verfasserin |4 aut | |
| 700 | 1 | |a Tung, Keith Ts |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Rosa S |e verfasserin |4 aut | |
| 700 | 1 | |a Tso, Winnie Wy |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Ian Ck |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Christina Sm |e verfasserin |4 aut | |
| 700 | 1 | |a Fong, Carol Hy |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Kwok Hung |e verfasserin |4 aut | |
| 700 | 1 | |a Yuen, Kwok Yung |e verfasserin |4 aut | |
| 700 | 1 | |a Ip, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Kwan, Mike Yw |e verfasserin |4 aut | |
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