The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways
© 2020. Published by The Company of Biologists Ltd..
Rho GTPases play a key role in various membrane trafficking processes. RhoU is an atypical small Rho GTPase related to Rac/Cdc42, which possesses unique N- and C-terminal domains that regulate its function and its subcellular localization. RhoU localizes at the plasma membrane, on endosomes and in cell adhesion structures where it governs cell signaling, differentiation and migration. However, despite its endomembrane localization, RhoU function in vesicular trafficking has been unexplored. Here, we identified intersectins (ITSNs) as new binding partners for RhoU and showed that the second PxxP motif at the N terminus of RhoU mediated interactions with the SH3 domains of ITSNs. To evaluate the function of RhoU and ITSNs in vesicular trafficking, we used fluorescent transferrin as a cargo for uptake experiments. We showed that silencing of either RhoU or ITSN2, but not ITSN1, increased transferrin accumulation in early endosomes, resulting from a defect in fast vesicle recycling. Concomitantly, RhoU and ITSN2 colocalized to a subset of Rab4-positive vesicles, suggesting that a RhoU-ITSN2 interaction may occur on fast recycling endosomes to regulate the fate of vesicular cargos.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:133 |
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| Enthalten in: |
Journal of cell science - 133(2020), 16 vom: 27. Aug. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gubar, Olga [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.06.2021 Date Revised 29.08.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1242/jcs.234104 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 4 | |a The atypical Rho GTPase RhoU interacts with intersectin-2 to regulate endosomal recycling pathways |
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| 500 | |a Date Revised 29.08.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020. Published by The Company of Biologists Ltd. | ||
| 520 | |a Rho GTPases play a key role in various membrane trafficking processes. RhoU is an atypical small Rho GTPase related to Rac/Cdc42, which possesses unique N- and C-terminal domains that regulate its function and its subcellular localization. RhoU localizes at the plasma membrane, on endosomes and in cell adhesion structures where it governs cell signaling, differentiation and migration. However, despite its endomembrane localization, RhoU function in vesicular trafficking has been unexplored. Here, we identified intersectins (ITSNs) as new binding partners for RhoU and showed that the second PxxP motif at the N terminus of RhoU mediated interactions with the SH3 domains of ITSNs. To evaluate the function of RhoU and ITSNs in vesicular trafficking, we used fluorescent transferrin as a cargo for uptake experiments. We showed that silencing of either RhoU or ITSN2, but not ITSN1, increased transferrin accumulation in early endosomes, resulting from a defect in fast vesicle recycling. Concomitantly, RhoU and ITSN2 colocalized to a subset of Rab4-positive vesicles, suggesting that a RhoU-ITSN2 interaction may occur on fast recycling endosomes to regulate the fate of vesicular cargos | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Atypical Rho GTPases | |
| 650 | 4 | |a Intersectins | |
| 650 | 4 | |a Proline-rich domains | |
| 650 | 4 | |a SH3 domains | |
| 650 | 4 | |a Vesicle recycling | |
| 650 | 7 | |a Adaptor Proteins, Vesicular Transport |2 NLM | |
| 650 | 7 | |a intersectin 1 |2 NLM | |
| 650 | 7 | |a rho GTP-Binding Proteins |2 NLM | |
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| 700 | 1 | |a Croisé, Pauline |e verfasserin |4 aut | |
| 700 | 1 | |a Kropyvko, Sergii |e verfasserin |4 aut | |
| 700 | 1 | |a Gryaznova, Tetyana |e verfasserin |4 aut | |
| 700 | 1 | |a Tóth, Petra |e verfasserin |4 aut | |
| 700 | 1 | |a Blangy, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Vitale, Nicolas |e verfasserin |4 aut | |
| 700 | 1 | |a Rynditch, Alla |e verfasserin |4 aut | |
| 700 | 1 | |a Gasman, Stéphane |e verfasserin |4 aut | |
| 700 | 1 | |a Ory, Stéphane |e verfasserin |4 aut | |
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