Enhanced Effect of Hyaluronan and Elastin Synthesis in Fibroblasts Through Lipopolysaccharide-activated Macrophages

Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved..

BACKGROUND/AIM: The functions of macrophages change in response to environmental factors such as lipopolysaccharide (LPS). LPS derived from Pantoea agglomerans (LPSp) is involved in macrophage activation and tissue repair when administered dermally. LPSp-activated macrophages may be useful for restoring and maintaining homeostasis of the skin.

MATERIALS AND METHODS: Phorbol myristate acetate-treated human monocytes (THP-1 cells) were activated with LPSp. The medium of LPSp-activated THP-1 cells was added to normal human dermal fibroblasts (NHDF cells). After 24 h, the expression of hyaluronan (HA) synthase (HAS)2, hyaluronidase (HYAL)1, and tropoelastin in NHDF cells was analyzed using quantitative real-time PCR.

RESULTS: The expression of HAS2 and tropoelastin was significantly increased, but that of HYAL1 was significantly decreased. It was demonstrated that the abilities of HA and elastin synthesis in NHDF cells increased through LPSp-activated THP-1 cells.

CONCLUSION: LPSp-activated macrophages may be useful for enhancing the abilities of HA and elastin synthesis in fibroblasts, subsequently improving dysfunction and reducing various age-related disorders.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:40

Enthalten in:

Anticancer research - 40(2020), 8 vom: 19. Aug., Seite 4681-4685

Sprache:

Englisch

Beteiligte Personen:

Honda, Teruko [VerfasserIn]
Tsutsui, Shoko [VerfasserIn]
Kohchi, Chie [VerfasserIn]
Inagawa, Hiroyuki [VerfasserIn]

Links:

Volltext

Themen:

9004-61-9
9007-58-3
Elastin
Fibroblast
Hyaluronan
Hyaluronic Acid
Journal Article
Lipopolysaccharide
Lipopolysaccharides
Macrophage
Monocyte

Anmerkungen:

Date Completed 10.08.2020

Date Revised 10.08.2020

published: Print

Citation Status MEDLINE

doi:

10.21873/anticanres.14467

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM313034133