Relationship between artificial cerebrospinal fluid oxygenation, slice depth and tissue performance in submerged brain slice experiments
Copyright © 2020 Elsevier B.V. All rights reserved..
One of the challenges for slice experimentalists is achieving optimal tissue oxygenation. One area that has not been addressed in submerged slices is the relationship between oxygenation of the artificial cerebrospinal fluid, slice depth and tissue performance. In this study we varied the depth of slice submersion, measured the oxygen profile in the solution and related these to slice activity in the form of spontaneous population events. While the oxygen profile curves were qualitatively similar (peaking approximately 1.7 mm below the solution surface), the average oxygen content was highly variable and correlated strongly with slice depth (R2 = 39%, p < 0.0001). The solution oxygen content was positively correlated with the frequency of population events (R2 = 21%, p < 0.0001). Optimum oxygenation and tissue performance were achieved with slices submerged at least 1.7 mm below the solution surface. In conclusion, in brain slice experiments it is important to measure and control the depth of slice submersion to maximise tissue oxygenation and reduce output variation between trials.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:736 |
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Enthalten in: |
Neuroscience letters - 736(2020) vom: 25. Sept., Seite 135275 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Voss, Logan J [VerfasserIn] |
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Links: |
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Themen: |
Brain |
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Anmerkungen: |
Date Completed 30.04.2021 Date Revised 30.04.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.neulet.2020.135275 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM312931581 |
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520 | |a One of the challenges for slice experimentalists is achieving optimal tissue oxygenation. One area that has not been addressed in submerged slices is the relationship between oxygenation of the artificial cerebrospinal fluid, slice depth and tissue performance. In this study we varied the depth of slice submersion, measured the oxygen profile in the solution and related these to slice activity in the form of spontaneous population events. While the oxygen profile curves were qualitatively similar (peaking approximately 1.7 mm below the solution surface), the average oxygen content was highly variable and correlated strongly with slice depth (R2 = 39%, p < 0.0001). The solution oxygen content was positively correlated with the frequency of population events (R2 = 21%, p < 0.0001). Optimum oxygenation and tissue performance were achieved with slices submerged at least 1.7 mm below the solution surface. In conclusion, in brain slice experiments it is important to measure and control the depth of slice submersion to maximise tissue oxygenation and reduce output variation between trials | ||
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