Helicobacter pylori infection modulates endogenous hydrogen sulfide production in gastric cancer AGS cells

© 2020 John Wiley & Sons Ltd..

BACKGROUND: Persistent Helicobacter pylori infection induces gastric mucosal atrophy, which is a precancerous condition. Hydrogen sulfide (H2 S), a gaseous biological transmitter, has been implicated in both the physiological functions of the gastrointestinal tract and its diseases. To understand gastric epithelial cell response against H pylori infection, we investigated the metabolic changes of gastric cancer cells co-cultured with H pylori and observed the modulation of endogenous H2 S production.

MATERIALS AND METHODS: Gastric cancer AGS cells were co-cultured with an H pylori standard strain possessing bacterial virulence factor CagA (ATCC 43504) and a strain without CagA (ATCC 51932). Three hours after inoculation, the cells were subjected to metabolomics analysis using gas chromatography-tandem mass spectrometry (GC-MS/MS). Orthogonal projections to latent structures discriminant analysis (OPLS-DA) and pathway analysis were performed. In addition, intracellular H2 S levels were measured by using HSip-1 fluorescent probe.

RESULTS: Results of OPLS-DA showed a significant difference between the metabolism of untreated control cells and cells inoculated with the H pylori strains ATCC 51932 or ATCC 43504, mainly due to 45 metabolites. Pathway analysis with the selected metabolites indicated that methionine metabolism, which is related to H2 S production, was the most frequently altered pathway. H pylori-inoculated cells produced more endogenous H2 S than control cells. Moreover, ATCC 43504-inoculated cells produced less H2 S than ATCC 51932-inoculated cells.

CONCLUSIONS: H pylori infection modulates endogenous H2 S production in AGS cells, suggesting that H2 S might be one of the bioactive molecules involved in the biological mechanisms of gastric mucosal disease including mucosal atrophy.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:25

Enthalten in:

Helicobacter - 25(2020), 5 vom: 26. Okt., Seite e12732

Sprache:

Englisch

Beteiligte Personen:

Kawahara, Yoshinari [VerfasserIn]
Hirashita, Yuka [VerfasserIn]
Tamura, Chikako [VerfasserIn]
Kudo, Yoko [VerfasserIn]
Sakai, Kumiko [VerfasserIn]
Togo, Kazumi [VerfasserIn]
Fukuda, Kensuke [VerfasserIn]
Matsunari, Osamu [VerfasserIn]
Okamoto, Kazuhisa [VerfasserIn]
Ogawa, Ryo [VerfasserIn]
Mizukami, Kazuhiro [VerfasserIn]
Okimoto, Tadayoshi [VerfasserIn]
Kodama, Masaaki [VerfasserIn]
Murakami, Kazunari [VerfasserIn]

Links:

Volltext

Themen:

Antigens, Bacterial
Bacterial Proteins
Cytotoxin-associated gene A
Helicobacter pylori
Hydrogen Sulfide
Hydrogen sulfide
Journal Article
Metabolomics
YY9FVM7NSN

Anmerkungen:

Date Completed 02.07.2021

Date Revised 02.07.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/hel.12732

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM312890567