No HIV-1 molecular evolution on long-term antiretroviral therapy initiated during primary HIV-1 infection

OBJECTIVE: Most studies about HIV-1 molecular evolution have shown the lack of viral evolution on effective antiretroviral therapy (ART), although controversial results have been documented. We therefore aimed to look for evidence of HIV-1 evolution in patients who initiated ART at the time of primary HIV-1 infection (PHI).

DESIGN: We included retrospectively 20 patients diagnosed at PHI, treated at the time of acute infection and with subsequent effective long-term suppressive ART (HIV viral load <20 copies/ml during at least 5 years without any blips).

METHODS: Longitudinal blood samples were deep sequenced using Illumina Miseq. Drug-resistance-associated mutations were retained at 2% cutoff and interpreted using the latest Agence Nationale de Recherches sur le Sida et les Hépatites Virales resistance algorithm. Viral evolution was established when temporal structure on maximum-likelihood phylogenetic tree and significant change over time of HIV-1 genetic diversity measured as the average pairwise distance was observed.

RESULTS: Emergences or disappearances of drug-resistance-associated mutations were detected in the blood cells during follow-up despite sustained virological control. In all patients, tree topologies showed an absence of segregation between sequences and blood viral populations from all time-points were intermingled. Comparison of the average pairwise distance showed the absence of significant viral diversity at the time of primary infection and afterwards during 5 years of full virological control under ART.

CONCLUSION: Despite a slight variation of minority resistance-associated mutation variants, there was no clear evidence of viral evolution during a prolonged period of time in this population of highly controlled adult patients treated at time of PHI.

Errataetall:

CommentIn: AIDS. 2020 Oct 1;34(12):1855-1856. - PMID 32889855

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:34

Enthalten in:

AIDS (London, England) - 34(2020), 12 vom: 01. Okt., Seite 1745-1753

Sprache:

Englisch

Beteiligte Personen:

Abdi, Basma [VerfasserIn]
Nguyen, Thuy [VerfasserIn]
Brouillet, Sophie [VerfasserIn]
Desire, Nathalie [VerfasserIn]
Sayon, Sophie [VerfasserIn]
Wirden, Marc [VerfasserIn]
Jary, Aude [VerfasserIn]
Achaz, Guillaume [VerfasserIn]
Assoumou, Lambert [VerfasserIn]
Palich, Romain [VerfasserIn]
Simon, Anne [VerfasserIn]
Tubiana, Roland [VerfasserIn]
Valantin, Marc-Antoine [VerfasserIn]
Katlama, Christine [VerfasserIn]
Calvez, Vincent [VerfasserIn]
Marcelin, Anne-Geneviève [VerfasserIn]
Soulie, Cathia [VerfasserIn]

Links:

Volltext

Themen:

Anti-HIV Agents
Journal Article

Anmerkungen:

Date Completed 18.02.2021

Date Revised 06.05.2021

published: Print

CommentIn: AIDS. 2020 Oct 1;34(12):1855-1856. - PMID 32889855

Citation Status MEDLINE

doi:

10.1097/QAD.0000000000002629

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM312706235