Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture
The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and control transport of macromolecules between the two compartments. Recently, it has been shown that the axial distance between the inner nuclear membrane and the cytoplasmic side of the NPC can be measured using dual-color metal-induced energy transfer (MIET). This chapter focuses on experimental aspects of this method and discusses the details of data analysis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2175 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2175(2020) vom: 17., Seite 33-45 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chizhik, Alexey I [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 17.03.2021 Date Revised 17.03.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-0763-3_4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM312578040 |
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| 520 | |a The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and control transport of macromolecules between the two compartments. Recently, it has been shown that the axial distance between the inner nuclear membrane and the cytoplasmic side of the NPC can be measured using dual-color metal-induced energy transfer (MIET). This chapter focuses on experimental aspects of this method and discusses the details of data analysis | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Metal-induced energy transfer | |
| 650 | 4 | |a Nuclear envelope | |
| 650 | 4 | |a Nuclear pore complex | |
| 650 | 4 | |a Optical microscopy | |
| 650 | 4 | |a Plasmonics | |
| 650 | 4 | |a Super-resolution microscopy | |
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| 650 | 7 | |a Membrane Proteins |2 NLM | |
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| 650 | 7 | |a ran-binding protein 2 |2 NLM | |
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| 700 | 1 | |a Ruhlandt, Daja |e verfasserin |4 aut | |
| 700 | 1 | |a Pfaff, Janine |e verfasserin |4 aut | |
| 700 | 1 | |a Karedla, Narain |e verfasserin |4 aut | |
| 700 | 1 | |a Gregor, Ingo |e verfasserin |4 aut | |
| 700 | 1 | |a Kehlenbach, Ralph H |e verfasserin |4 aut | |
| 700 | 1 | |a Enderlein, Jörg |e verfasserin |4 aut | |
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