Frequent Recombination Events in Leishmania donovani : Mining Population Data

The Leishmania donovani species complex consists of all L. donovani and L. infantum strains mainly responsible for visceral leishmaniasis (VL). It was suggested that genome rearrangements in Leishmania spp. occur very often, thus enabling parasites to adapt to the different environmental conditions. Some of these rearrangements may be directly linked to the virulence or explain the reduced efficacy of antimonial drugs in some isolates. In the current study, we focused on a large-scale analysis of putative gene conversion events using publicly available datasets. Previous population study of L. donovani suggested that population variability of L. donovani is relatively low, however the authors used masking procedures and strict read selection criteria. We decided to re-analyze DNA-seq data without masking sequences, because we were interested in the most dynamic fraction of the genome. The majority of samples have an excess of putative gene conversion/recombination events in the noncoding regions, however we found an overall excess of putative intrachromosomal gene conversion/recombination in the protein coding genes, compared to putative interchromosomal gene conversion/recombination events.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:9

Enthalten in:

Pathogens (Basel, Switzerland) - 9(2020), 7 vom: 15. Juli

Sprache:

Englisch

Beteiligte Personen:

Rogozin, Igor B [VerfasserIn]
Charyyeva, Arzuv [VerfasserIn]
Sidorenko, Ivan A [VerfasserIn]
Babenko, Vladimir N [VerfasserIn]
Yurchenko, Vyacheslav [VerfasserIn]

Links:

Volltext

Themen:

Concerted evolution
Gene conversion
Journal Article
Leishmania donovani species complex
Whole-genome sequencing

Anmerkungen:

Date Revised 28.09.2020

published: Electronic

Citation Status PubMed-not-MEDLINE

doi:

10.3390/pathogens9070572

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM312560141