In vivo and in vitro radiotherapy increased dentin enzymatic activity
Copyright © 2020 Elsevier Ltd. All rights reserved..
OBJECTIVES: The present study investigated the effects of in vitro and in-vivo radiotherapy on endogenous enzymatic activity in dentin using gelatin zymography and in-situ zymography.
METHODS: Gelatin zymographic assays were performed on protein extracts obtained from dentin powder of sound non-irradiated (NRT), in vitro irradiated (VTRT) and in vivo irradiated (VIRT) human teeth. Their proteolytic activities were quantified using band densitometric evaluation. For in-situ zymography, dentin specimens from NRT, VIRT and VTRT were covered with fluorescein-conjugated gelatin and examined with confocal laser-scanning microscopy. Fluorescence intensity emitted by the hydrolyzed fluorescein-conjugated gelatin was quantified and statistically analyzed. In-situ zymography data were statistically analyzed using Kruskal-Wallis ANOVA and Dunn's multiple comparison procedures (α = 0.05).
RESULTS: No difference between in vitro and in vivo radiotherapy treatment was found. Both VTRT and VIRT groups showed increase in MMP-9 expression when compared to NRT group. Significant increases (p < 0.05) in gelatinolytic activity (26 % for VTRT; 55 % for VIRT) were observed when compared to the NRT group.
CONCLUSION: Radiotherapy increase endogenous enzymatic activity in non-restored dentin.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:100 |
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| Enthalten in: |
Journal of dentistry - 100(2020) vom: 01. Sept., Seite 103429 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cunha, Sandra Ribeiro [VerfasserIn] |
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| Links: |
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| Themen: |
Dentin-Bonding Agents |
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| Anmerkungen: |
Date Completed 19.02.2021 Date Revised 19.02.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jdent.2020.103429 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM312500459 |
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| 100 | 1 | |a Cunha, Sandra Ribeiro |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a In vivo and in vitro radiotherapy increased dentin enzymatic activity |
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| 500 | |a Date Revised 19.02.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Ltd. All rights reserved. | ||
| 520 | |a OBJECTIVES: The present study investigated the effects of in vitro and in-vivo radiotherapy on endogenous enzymatic activity in dentin using gelatin zymography and in-situ zymography | ||
| 520 | |a METHODS: Gelatin zymographic assays were performed on protein extracts obtained from dentin powder of sound non-irradiated (NRT), in vitro irradiated (VTRT) and in vivo irradiated (VIRT) human teeth. Their proteolytic activities were quantified using band densitometric evaluation. For in-situ zymography, dentin specimens from NRT, VIRT and VTRT were covered with fluorescein-conjugated gelatin and examined with confocal laser-scanning microscopy. Fluorescence intensity emitted by the hydrolyzed fluorescein-conjugated gelatin was quantified and statistically analyzed. In-situ zymography data were statistically analyzed using Kruskal-Wallis ANOVA and Dunn's multiple comparison procedures (α = 0.05) | ||
| 520 | |a RESULTS: No difference between in vitro and in vivo radiotherapy treatment was found. Both VTRT and VIRT groups showed increase in MMP-9 expression when compared to NRT group. Significant increases (p < 0.05) in gelatinolytic activity (26 % for VTRT; 55 % for VIRT) were observed when compared to the NRT group | ||
| 520 | |a CONCLUSION: Radiotherapy increase endogenous enzymatic activity in non-restored dentin | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Endogenous enzymatic activity | |
| 650 | 4 | |a Head and neck cancer | |
| 650 | 4 | |a Radiotherapy | |
| 650 | 4 | |a Zymography | |
| 650 | 7 | |a Dentin-Bonding Agents |2 NLM | |
| 650 | 7 | |a Resin Cements |2 NLM | |
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| 700 | 1 | |a Maravic, Tatjana |e verfasserin |4 aut | |
| 700 | 1 | |a Comba, Allegra |e verfasserin |4 aut | |
| 700 | 1 | |a Ramos, Pedro Augusto |e verfasserin |4 aut | |
| 700 | 1 | |a Tay, Franklin R |e verfasserin |4 aut | |
| 700 | 1 | |a Pashley, David H |e verfasserin |4 aut | |
| 700 | 1 | |a Fregnani, Eduardo Rodrigues |e verfasserin |4 aut | |
| 700 | 1 | |a Aranha, Ana Cecília |e verfasserin |4 aut | |
| 700 | 1 | |a Mazzoni, Annalisa |e verfasserin |4 aut | |
| 700 | 1 | |a Breschi, Lorenzo |e verfasserin |4 aut | |
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