Genotyping and phylogenic study of Acanthamoeba isolates from human keratitis and swimming pool water samples in Iran
© 2020 Published by Elsevier Ltd on behalf of World Federation of Parasitologists..
OBJECTIVE: Acanthamoeba keratitis cause severe corneal infection and lead to poor vision and blindness. This disease is caused by a unicellular amphizoic protozoon called Acanthamoeba spp. that present in different environments. This study aimed to represent the existence and genotyping of Acanthamoeba spp. in patients with keratitis and swimming pool water (SPW) in Tehran Province, Central Iran.
METHODS: In this descriptive study, 56 clinical samples were collected from patients with keratitis and 30 water samples were collected from different swimming pools in Tehran Province. All samples were examined based on the morphological and molecular techniques. The genotypes were determined by sequencing the partial of 18S rRNA gene.
RESULTS: Of 56 clinical (corneal) and 30 environmental (SPW) samples, 30.3% and 40.0% were positive for Acanthamoeba spp., respectively. According to sequencing analysis, 94.1% of amoebic keratitis isolates were belonged to T4 genotype and only one (5.8%) isolate was belonged to T11 genotype. All genotypes were detected from SPW samples were identified as T4 genotype.
CONCLUSION: According to our results, use of contact lens and swimming in pool poses the major risk factor for amoebic keratitis in the studied area (Tehran). Moreover, T4 genotype was the predominant genotype of human keratitis and swimming pool samples there. Consequently, essential and practical measures are urgently needed to prevent subjects against this ocular seriously disease.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
|---|---|
| Enthalten in: |
Parasite epidemiology and control - 11(2020) vom: 15. Nov., Seite e00164 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Esboei, Bahman Rahimi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Acanthamoeba |
|---|
| Anmerkungen: |
Date Revised 15.04.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1016/j.parepi.2020.e00164 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM312476736 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM312476736 | ||
| 003 | DE-627 | ||
| 005 | 20231225144509.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.parepi.2020.e00164 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1041.xml |
| 035 | |a (DE-627)NLM312476736 | ||
| 035 | |a (NLM)32671234 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Esboei, Bahman Rahimi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genotyping and phylogenic study of Acanthamoeba isolates from human keratitis and swimming pool water samples in Iran |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 15.04.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2020 Published by Elsevier Ltd on behalf of World Federation of Parasitologists. | ||
| 520 | |a OBJECTIVE: Acanthamoeba keratitis cause severe corneal infection and lead to poor vision and blindness. This disease is caused by a unicellular amphizoic protozoon called Acanthamoeba spp. that present in different environments. This study aimed to represent the existence and genotyping of Acanthamoeba spp. in patients with keratitis and swimming pool water (SPW) in Tehran Province, Central Iran | ||
| 520 | |a METHODS: In this descriptive study, 56 clinical samples were collected from patients with keratitis and 30 water samples were collected from different swimming pools in Tehran Province. All samples were examined based on the morphological and molecular techniques. The genotypes were determined by sequencing the partial of 18S rRNA gene | ||
| 520 | |a RESULTS: Of 56 clinical (corneal) and 30 environmental (SPW) samples, 30.3% and 40.0% were positive for Acanthamoeba spp., respectively. According to sequencing analysis, 94.1% of amoebic keratitis isolates were belonged to T4 genotype and only one (5.8%) isolate was belonged to T11 genotype. All genotypes were detected from SPW samples were identified as T4 genotype | ||
| 520 | |a CONCLUSION: According to our results, use of contact lens and swimming in pool poses the major risk factor for amoebic keratitis in the studied area (Tehran). Moreover, T4 genotype was the predominant genotype of human keratitis and swimming pool samples there. Consequently, essential and practical measures are urgently needed to prevent subjects against this ocular seriously disease | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Acanthamoeba | |
| 650 | 4 | |a Genotype | |
| 650 | 4 | |a Keratitis | |
| 650 | 4 | |a Swimming pool water | |
| 650 | 4 | |a T4 | |
| 700 | 1 | |a Fakhar, Mahdi |e verfasserin |4 aut | |
| 700 | 1 | |a Saberi, Reza |e verfasserin |4 aut | |
| 700 | 1 | |a Barati, Mohammad |e verfasserin |4 aut | |
| 700 | 1 | |a Moslemi, Masoumeh |e verfasserin |4 aut | |
| 700 | 1 | |a Hassannia, Hadi |e verfasserin |4 aut | |
| 700 | 1 | |a Dadimoghadam, Yousef |e verfasserin |4 aut | |
| 700 | 1 | |a Jalallou, Nahid |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Parasite epidemiology and control |d 2016 |g 11(2020) vom: 15. Nov., Seite e00164 |w (DE-627)NLM262542021 |x 2405-6731 |7 nnns |
| 773 | 1 | 8 | |g volume:11 |g year:2020 |g day:15 |g month:11 |g pages:e00164 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.parepi.2020.e00164 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 11 |j 2020 |b 15 |c 11 |h e00164 | ||