Identification and characterization of a female gametophyte defect in sdk1-7 +/- abi3-6 +/- heterozygotes of Arabidopsis thaliana

Successful reproduction in angiosperms is dependent on the highly synchronous development of their male and female gametophytes and the ensuing fusion of the gametes from these reproductive tissue types. When crossing a T-DNA insertion line sdk1-7-/-(Salk_024564), one of the S-domain receptor kinases involved in ABA responses with a fast neutron deletion line abi3-6-/-, the F1 heterozygotes (sdk1-7+/-abi3-6 +/-) displayed 50% ovule abortion suggesting a likely gametophytic defects. We identified and characterized an early stage female gametophyte developmental defect in the heterozygous mutant ovules. Recombination frequency analysis of the F2 progenies from selfed heterozygotes revealed a possible pseudo-linkage of sdk1-7 and abi3-6 suggesting a reciprocal translocation event in the heterozygote. Our study emphasizes the importance of robust analysis to distinguish gametophytic defect phenotypes caused by genetic interactions and that resulting from possible chromosomal translocation events.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

Plant signaling & behavior - 15(2020), 8 vom: 02. Aug., Seite 1780038

Sprache:

Englisch

Beteiligte Personen:

Sankaranarayanan, Subramanian [VerfasserIn]
Jamshed, Muhammad [VerfasserIn]
Delmas, Frédéric [VerfasserIn]
Yeung, Edward C [VerfasserIn]
Samuel, Marcus A [VerfasserIn]

Links:

Volltext

Themen:

Abi3
Arabidopsis Proteins
B3 domain transcription factor
Fast neutron deletion
Female gametophyte (FG)
Heterozygote
Journal Article
Pseudo-linkage
Reciprocal translocation
Research Support, Non-U.S. Gov't
S-domain receptor kinase
T-DNA insertion

Anmerkungen:

Date Completed 28.05.2021

Date Revised 09.11.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1080/15592324.2020.1780038

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM312339488