A functionally neutral single chain antibody to measure beta-1 integrin uptake and recycling
© 2020 The Authors. Traffic published by John Wiley & Sons Ltd..
Integrin-mediated cell adhesion and signaling are critical for many physiological processes. The dynamic turnover of integrins and their associated adhesion complexes through endocytic and recycling pathways has emerged as an important mechanism for controlling cell migration and invasion in cancer. Thus, the regulation of integrin trafficking and how this may be altered by disease-specific molecular mechanisms has generated considerable interest. However, current tools available to study integrin trafficking may cause artifacts and/or do not provide adequate kinetic information. Here, we report the generation of a functionally neutral and monovalent single chain antibody to quantitatively and qualitatively measure β1 integrin trafficking in cells. Our novel probe can be used in a variety of assays and allows for the biochemical characterization of rapid recycling of endogenous integrins. We also demonstrate its potential utility in live cell imaging, providing proof of principle to guide future integrin probe design.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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| Enthalten in: |
Traffic (Copenhagen, Denmark) - 21(2020), 9 vom: 15. Sept., Seite 590-602 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lakoduk, Ashley M [VerfasserIn] |
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| Links: |
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| Themen: |
Endocytosis |
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| Anmerkungen: |
Date Completed 27.04.2021 Date Revised 27.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/tra.12754 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311914926 |
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| 245 | 1 | 2 | |a A functionally neutral single chain antibody to measure beta-1 integrin uptake and recycling |
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| 500 | |a Date Revised 27.04.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 The Authors. Traffic published by John Wiley & Sons Ltd. | ||
| 520 | |a Integrin-mediated cell adhesion and signaling are critical for many physiological processes. The dynamic turnover of integrins and their associated adhesion complexes through endocytic and recycling pathways has emerged as an important mechanism for controlling cell migration and invasion in cancer. Thus, the regulation of integrin trafficking and how this may be altered by disease-specific molecular mechanisms has generated considerable interest. However, current tools available to study integrin trafficking may cause artifacts and/or do not provide adequate kinetic information. Here, we report the generation of a functionally neutral and monovalent single chain antibody to quantitatively and qualitatively measure β1 integrin trafficking in cells. Our novel probe can be used in a variety of assays and allows for the biochemical characterization of rapid recycling of endogenous integrins. We also demonstrate its potential utility in live cell imaging, providing proof of principle to guide future integrin probe design | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a endocytosis | |
| 650 | 4 | |a integrin | |
| 650 | 4 | |a recycling | |
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| 650 | 4 | |a trafficking | |
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| 700 | 1 | |a Kadlecova, Zuzana |e verfasserin |4 aut | |
| 700 | 1 | |a Schmid, Sandra L |e verfasserin |4 aut | |
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