Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver

Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in Kupffer cells (KCs) in mice. This technology is based on baker's yeast and allows gene manipulation in macrophages in a tissue-specific manner depending on the route of administration and the model that is used. GeRP administered by intravenous injection in mice are delivered to KCs. Therefore, using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by KCs in the regulation of liver function.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:2164

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 2164(2020) vom: 30., Seite 65-73

Sprache:

Englisch

Beteiligte Personen:

Tencerova, Michaela [VerfasserIn]

Links:

Volltext

Themen:

Diabetes
Gene silencing
Glucans
Inflammation
Insulin sensitivity
Intravenous administration
Journal Article
Kupffer cells
Mouse liver
Obesity
RNA, Small Interfering
RNAi delivery
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 11.03.2021

Date Revised 11.03.2021

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-0716-0704-6_8

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311858163