PEG-PtS2 nanosheet-based fluorescence biosensor for label-free human papillomavirus genotyping

A simple and efficient ultrasonication-assisted liquid exfoliation method is proposed to produce PtS2 nanosheets on a large scale and improve their dispersion in aqueous solution by surface polyethylene glycol modification. The interaction of polyethylene glycol-modified PtS2 (PEG-PtS2) nanosheets with fluorescent labeled DNA and the fluorescence quenching mechanism using FAM-labeled hpv16e6 gene fragment as a probe was investigated. The excitation and emission wavelengths were 468 and 517 nm, respectively. The fluorescence quenching mechanism of PEG-PtS2 nanosheets for double-stranded DNA (dsDNA) might stem from the static quenching effect. Based on the difference in fluorescence quenching capability of PEG-PtS2 nanosheets in fluorescent probe tagged single-stranded DNA (ssDNA) and dsDNA, a mix-and-detect method was proposed for determination of DNA. Without the need for probe immobilization and tedious washing steps, the genotyping of human papillomavirus (HPV) was easily achieved. The limit of detection was calculated to 0.44 nM, showing a good linear range within 0.05-10 nM. We believe this biosensor provides opportunities to develop a simple and low-cost strategy for molecular diagnostics. Graphical abstract.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:187

Enthalten in:

Mikrochimica acta - 187(2020), 7 vom: 29. Juni, Seite 408

Sprache:

Englisch

Beteiligte Personen:

Zhang, Wenxian [VerfasserIn]
Li, Shenchang [VerfasserIn]
Li, Xiuping [VerfasserIn]
Liu, Man [VerfasserIn]
Cui, Tianyu [VerfasserIn]
Fu, Huayang [VerfasserIn]
Yang, Mengsu [VerfasserIn]
Zhong, Wenying [VerfasserIn]
Xu, Bo [VerfasserIn]
Yue, Wanqing [VerfasserIn]

Links:

Volltext

Themen:

3WJQ0SDW1A
DNA, Viral
DNA fluorescence biosensor
Fluorescent Dyes
Journal Article
Platinum Compounds
Platinum disulfide nanosheets
Polyethylene Glycols
Polyethylene glycol modification
Research Support, Non-U.S. Gov't
Ultrasonication-assisted liquid exfoliation method

Anmerkungen:

Date Completed 10.05.2021

Date Revised 10.05.2021

published: Electronic

Citation Status MEDLINE

doi:

10.1007/s00604-020-04383-8

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311798624