Rabbit model of Staphylococcus aureus implant-associated spinal infection
© 2020. Published by The Company of Biologists Ltd..
Post-surgical implant-associated spinal infection is a devastating complication commonly caused by Staphylococcus aureus Biofilm formation is thought to reduce penetration of antibiotics and immune cells, contributing to chronic and difficult-to-treat infections. A rabbit model of a posterior-approach spinal surgery was created, in which bilateral titanium pedicle screws were interconnected by a plate at the level of lumbar vertebra L6 and inoculated with a methicillin-resistant S.aureus (MRSA) bioluminescent strain. In vivo whole-animal bioluminescence imaging (BLI) and ex vivo bacterial cultures demonstrated a peak in bacterial burden by day 14, when wound dehiscence occurred. Structures suggestive of biofilm, visualized by scanning electron microscopy, were evident up to 56 days following infection. Infection-induced inflammation and bone remodeling were also monitored using 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) and computed tomography (CT). PET imaging signals were noted in the soft tissue and bone surrounding the implanted materials. CT imaging demonstrated marked bone remodeling and a decrease in dense bone at the infection sites. This rabbit model of implant-associated spinal infection provides a valuable preclinical in vivo approach to investigate the pathogenesis of implant-associated spinal infections and to evaluate novel therapeutics.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Disease models & mechanisms - 13(2020), 7 vom: 28. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gordon, Oren [VerfasserIn] |
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| Links: |
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| Themen: |
Bioluminescence |
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| Anmerkungen: |
Date Completed 23.08.2021 Date Revised 23.08.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1242/dmm.045385 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311651364 |
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| 520 | |a © 2020. Published by The Company of Biologists Ltd. | ||
| 520 | |a Post-surgical implant-associated spinal infection is a devastating complication commonly caused by Staphylococcus aureus Biofilm formation is thought to reduce penetration of antibiotics and immune cells, contributing to chronic and difficult-to-treat infections. A rabbit model of a posterior-approach spinal surgery was created, in which bilateral titanium pedicle screws were interconnected by a plate at the level of lumbar vertebra L6 and inoculated with a methicillin-resistant S.aureus (MRSA) bioluminescent strain. In vivo whole-animal bioluminescence imaging (BLI) and ex vivo bacterial cultures demonstrated a peak in bacterial burden by day 14, when wound dehiscence occurred. Structures suggestive of biofilm, visualized by scanning electron microscopy, were evident up to 56 days following infection. Infection-induced inflammation and bone remodeling were also monitored using 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) and computed tomography (CT). PET imaging signals were noted in the soft tissue and bone surrounding the implanted materials. CT imaging demonstrated marked bone remodeling and a decrease in dense bone at the infection sites. This rabbit model of implant-associated spinal infection provides a valuable preclinical in vivo approach to investigate the pathogenesis of implant-associated spinal infections and to evaluate novel therapeutics | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Implant-associated infection | |
| 650 | 4 | |a PET | |
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| 650 | 4 | |a Spinal infection | |
| 700 | 1 | |a Miller, Robert J |e verfasserin |4 aut | |
| 700 | 1 | |a Thompson, John M |e verfasserin |4 aut | |
| 700 | 1 | |a Ordonez, Alvaro A |e verfasserin |4 aut | |
| 700 | 1 | |a Klunk, Mariah H |e verfasserin |4 aut | |
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| 700 | 1 | |a Joyce, Daniel P |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz-Bedoya, Camilo A |e verfasserin |4 aut | |
| 700 | 1 | |a Miller, Lloyd S |e verfasserin |4 aut | |
| 700 | 1 | |a Jain, Sanjay K |e verfasserin |4 aut | |
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