Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
| Year of Publication: | 2020 |
| Contained in: | International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases Vol. 97 (2020), p. 66-68 |
| All journal articles: |
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| Language: |
English |
| Contributors: |
Alcoba-Florez, Julia
| Author González-Montelongo, Rafaela Íñigo-Campos, Antonio de Artola, Diego García-Martínez Gil-Campesino, Helena The Microbiology Technical Support Team Ciuffreda, Laura Valenzuela-Fernández, Agustín Flores, Carlos |
| Full text access: |
Electronic availability is being checked...
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| Links: |
Full Text (dx.doi.org) |
| Keywords: |
COVID-19
Journal Article RNA extraction SARS-CoV-2 diagnosis fast protocols sample treatment |
| Additional Keywords: |
Betacoronavirus Coronavirus Infections Diagnostic Tests, Routine Hot Temperature Humans Pandemics Pneumonia, Viral Real-Time Polymerase Chain Reaction |
| ISSN: |
1878-3511 |
| Note: | Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine |
| Notes: |
Date Completed 03.08.2020 Date Revised 03.08.2020 published: Print-Electronic Citation Status MEDLINE Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine |
| PMID: |
|
| Physical Description: |
Online-Ressource |
| ID (e.g. DOI, URN): |
10.1016/j.ijid.2020.05.099 |
Internes Format
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| 100 | 1 | |a Alcoba-Florez, Julia | |
| 245 | 1 | 0 | |a Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples |h Elektronische Ressource |
| 300 | |a Online-Ressource | ||
| 500 | |a Date Completed 03.08.2020 | ||
| 500 | |a Date Revised 03.08.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 500 | |a Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine | ||
| 520 | |a Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a OBJECTIVES: The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection | ||
| 520 | |a METHODS: We evaluated three methods based on direct nasopharyngeal swab viral transmission medium (VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) in a RNAsnapTM buffer | ||
| 520 | |a RESULTS: Although with a delay in cycle threshold compared to the gold-standard, we found consistent results in nasopharyngeal swab samples that were subject to a direct 70°C incubation for 10 min | ||
| 520 | |a CONCLUSIONS: Our findings provide valuable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests, thereby complementing standard diagnosis | ||
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| 653 | 2 | |a Betacoronavirus |6 D000073640 |a *genetics |6 Q000235 | |
| 653 | 2 | |a Coronavirus Infections |6 D018352 |a *diagnosis |6 Q000175 |a virology |6 Q000821 | |
| 653 | 2 | |a Diagnostic Tests, Routine |6 D003955 | |
| 653 | 2 | |a Hot Temperature |6 D006358 | |
| 653 | 2 | |a Humans |6 D006801 | |
| 653 | 2 | |a Pandemics |6 D058873 | |
| 653 | 2 | |a Pneumonia, Viral |6 D011024 |a *diagnosis |6 Q000175 |a virology |6 Q000821 | |
| 653 | 2 | |a Real-Time Polymerase Chain Reaction |6 D060888 | |
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| 655 | 7 | |a RNA extraction |2 gnd | |
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| 700 | 1 | |a González-Montelongo, Rafaela | |
| 700 | 1 | |a Íñigo-Campos, Antonio | |
| 700 | 1 | |a de Artola, Diego García-Martínez | |
| 700 | 1 | |a Gil-Campesino, Helena | |
| 700 | 3 | |a The Microbiology Technical Support Team | |
| 700 | 1 | |a Ciuffreda, Laura | |
| 700 | 1 | |a Valenzuela-Fernández, Agustín | |
| 700 | 1 | |a Flores, Carlos | |
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