Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved..
OBJECTIVES: The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection.
METHODS: We evaluated three methods based on direct nasopharyngeal swab viral transmission medium (VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) in a RNAsnapTM buffer.
RESULTS: Although with a delay in cycle threshold compared to the gold-standard, we found consistent results in nasopharyngeal swab samples that were subject to a direct 70°C incubation for 10 min.
CONCLUSIONS: Our findings provide valuable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests, thereby complementing standard diagnosis.
| Media Type: |
Electronic Article |
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| Year of Publication: |
2020 |
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| Contained In: |
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - Vol. 97 (2020), p. 66-68 |
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| Language: |
English |
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| Links: |
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| Notes: |
Date Completed 03.08.2020 Date Revised 27.01.2021 published: Print-Electronic Citation Status MEDLINE Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine |
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| Physical Description: |
Online-Ressource |
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| doi: |
10.1016/j.ijid.2020.05.099 |
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| PMID: |
32492531 |
| PPN (Catalogue-ID): |
NLM311615589 |
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| 100 | 1 | |a Alcoba-Florez, Julia | |
| 245 | 1 | 0 | |a Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples |h Elektronische Ressource |
| 300 | |a Online-Ressource | ||
| 500 | |a Date Completed 03.08.2020 | ||
| 500 | |a Date Revised 27.01.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 500 | |a Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine | ||
| 520 | |a Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a OBJECTIVES: The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection | ||
| 520 | |a METHODS: We evaluated three methods based on direct nasopharyngeal swab viral transmission medium (VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) in a RNAsnapTM buffer | ||
| 520 | |a RESULTS: Although with a delay in cycle threshold compared to the gold-standard, we found consistent results in nasopharyngeal swab samples that were subject to a direct 70°C incubation for 10 min | ||
| 520 | |a CONCLUSIONS: Our findings provide valuable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests, thereby complementing standard diagnosis | ||
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| 653 | 2 | |a COVID-19 |6 D000086382 | |
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| 700 | 1 | |a González-Montelongo, Rafaela | |
| 700 | 1 | |a Íñigo-Campos, Antonio | |
| 700 | 1 | |a de Artola, Diego García-Martínez | |
| 700 | 1 | |a Gil-Campesino, Helena | |
| 700 | 3 | |a The Microbiology Technical Support Team | |
| 700 | 1 | |a Ciuffreda, Laura | |
| 700 | 1 | |a Valenzuela-Fernández, Agustín | |
| 700 | 1 | |a Flores, Carlos | |
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