Age-Dependent Heterogeneity of Murine Olfactory Bulb Astrocytes

Copyright © 2020 Klein, Lohr and Droste..

Astrocytes have a high impact on the structure of the central nervous system, as they control neural activity, development, and plasticity. Heterogeneity of astrocytes has been shown before, but so far only a few studies have demonstrated heterogeneous morphology of astrocytes concerning aging. In this study, we examined morphologic differences of astrocyte subpopulations in adult mice and the progression of these differences with age. We surveyed astrocytes in olfactory bulb slices of mice aged 3 months, 1 year and 2 years (three animals each age group), based on their appearance in anti-GFAP immunostaining. Based on this data we established three different types of astrocytes: type I (stellate), type II (elliptic), and type III (squid-like). We found that with the advanced age of the mice, astrocytes grow in size and complexity. Major changes occurred between the ages of 3 months and 1 year, while between 1 and 2 years no significant development in cell size and complexity could be detected. Our results show that astrocytes in the olfactory bulb are heterogeneous and undergo morphological transformation until late adolescence but not upon senescence. Structural plasticity is further substantiated by the expression of vimentin in some astrocyte processes in all age groups.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:12

Enthalten in:

Frontiers in aging neuroscience - 12(2020) vom: 06., Seite 172

Sprache:

Englisch

Beteiligte Personen:

Klein, Marcel [VerfasserIn]
Lohr, Christian [VerfasserIn]
Droste, Damian [VerfasserIn]

Links:

Volltext

Themen:

Aging
Astrocyte
Cell morphology
Heterogeneity
Journal Article
Olfactory bulb
Sholl analysis

Anmerkungen:

Date Revised 28.09.2020

published: Electronic-eCollection

Citation Status PubMed-not-MEDLINE

doi:

10.3389/fnagi.2020.00172

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311601502