Replication origin location might contribute to genetic variability in Trypanosoma cruzi

BACKGROUND: DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown.

RESULTS: Here we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T. cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1).

CONCLUSIONS: These findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:21

Enthalten in:

BMC genomics - 21(2020), 1 vom: 22. Juni, Seite 414

Sprache:

Englisch

Beteiligte Personen:

de Araujo, Christiane Bezerra [VerfasserIn]
da Cunha, Julia Pinheiro Chagas [VerfasserIn]
Inada, Davi Toshio [VerfasserIn]
Damasceno, Jeziel [VerfasserIn]
Lima, Alex Ranieri Jerônimo [VerfasserIn]
Hiraiwa, Priscila [VerfasserIn]
Marques, Catarina [VerfasserIn]
Gonçalves, Evonnildo [VerfasserIn]
Nishiyama-Junior, Milton Yutaka [VerfasserIn]
McCulloch, Richard [VerfasserIn]
Elias, Maria Carolina [VerfasserIn]

Links:

Volltext

Themen:

DGF-1
DNA, Protozoan
Genetic variability
Journal Article
Replication origins
Trypanosoma cruzi

Anmerkungen:

Date Completed 29.01.2021

Date Revised 17.03.2021

published: Electronic

Citation Status MEDLINE

doi:

10.1186/s12864-020-06803-8

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311497632