Identification of a Neutralizing Monoclonal Antibody That Recognizes a Unique Epitope on Domain III of the Envelope Protein of Tembusu Virus
Domain III of the envelope protein (EDIII) is the major target of flavivirus neutralizing antibody. To date, little is known regarding antibody-mediated neutralization of Tembusu virus (TMUV), a novel flavivirus emerging in duck in 2010. Here, a novel monoclonal antibody (MAb), designated 12F11, was prepared by immunization of mice with recombinant EDIII (rEDIII) protein. Using virus neutralization test, 12F11 in undiluted ascites neutralized the TMUV infectivity to induce the development of cytopathic effects in BHK-21 cells, indicating that 12F11 exhibits a neutralizing activity. The neutralizing activity of 12F11 was confirmed by plaque reduction neutralization test, in which 12F11 reduced significantly the number of plaques produced by TMUV in BHK-21 cells. Western blot analyses of a series of truncated rEDIII proteins showed that the epitope recognized by 12F11 includes amino acids between residues 8 and 77 of EDIII protein. Function analysis demonstrated that 12F11 neutralizes TMUV infection at virus adsorption and at a step after adsorption to a certain extent. The present study provides an important step towards elucidating antibody-mediated neutralization of TMUV.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2020 |
---|---|
Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
---|---|
Enthalten in: |
Viruses - 12(2020), 6 vom: 15. Juni |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Qu, Shenghua [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 19.02.2021 Date Revised 19.02.2021 published: Electronic Citation Status MEDLINE |
---|
doi: |
10.3390/v12060647 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM311280021 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM311280021 | ||
003 | DE-627 | ||
005 | 20231225141920.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3390/v12060647 |2 doi | |
028 | 5 | 2 | |a pubmed24n1037.xml |
035 | |a (DE-627)NLM311280021 | ||
035 | |a (NLM)32549221 | ||
035 | |a (PII)E647 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Qu, Shenghua |e verfasserin |4 aut | |
245 | 1 | 0 | |a Identification of a Neutralizing Monoclonal Antibody That Recognizes a Unique Epitope on Domain III of the Envelope Protein of Tembusu Virus |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 19.02.2021 | ||
500 | |a Date Revised 19.02.2021 | ||
500 | |a published: Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Domain III of the envelope protein (EDIII) is the major target of flavivirus neutralizing antibody. To date, little is known regarding antibody-mediated neutralization of Tembusu virus (TMUV), a novel flavivirus emerging in duck in 2010. Here, a novel monoclonal antibody (MAb), designated 12F11, was prepared by immunization of mice with recombinant EDIII (rEDIII) protein. Using virus neutralization test, 12F11 in undiluted ascites neutralized the TMUV infectivity to induce the development of cytopathic effects in BHK-21 cells, indicating that 12F11 exhibits a neutralizing activity. The neutralizing activity of 12F11 was confirmed by plaque reduction neutralization test, in which 12F11 reduced significantly the number of plaques produced by TMUV in BHK-21 cells. Western blot analyses of a series of truncated rEDIII proteins showed that the epitope recognized by 12F11 includes amino acids between residues 8 and 77 of EDIII protein. Function analysis demonstrated that 12F11 neutralizes TMUV infection at virus adsorption and at a step after adsorption to a certain extent. The present study provides an important step towards elucidating antibody-mediated neutralization of TMUV | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Tembusu virus | |
650 | 4 | |a antibody-mediated neutralization | |
650 | 4 | |a duck | |
650 | 4 | |a epitope | |
650 | 4 | |a monoclonal antibody | |
650 | 4 | |a neutralizing antibody | |
650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
650 | 7 | |a Antibodies, Neutralizing |2 NLM | |
650 | 7 | |a Epitopes |2 NLM | |
650 | 7 | |a Viral Envelope Proteins |2 NLM | |
700 | 1 | |a Wang, Xiaoyan |e verfasserin |4 aut | |
700 | 1 | |a Yang, Lixin |e verfasserin |4 aut | |
700 | 1 | |a Lv, Junfeng |e verfasserin |4 aut | |
700 | 1 | |a Meng, Runze |e verfasserin |4 aut | |
700 | 1 | |a Dai, Weiqian |e verfasserin |4 aut | |
700 | 1 | |a Li, Qingxiangzi |e verfasserin |4 aut | |
700 | 1 | |a Liu, Huicong |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Bing |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Dabing |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Viruses |d 2009 |g 12(2020), 6 vom: 15. Juni |w (DE-627)NLM192382764 |x 1999-4915 |7 nnns |
773 | 1 | 8 | |g volume:12 |g year:2020 |g number:6 |g day:15 |g month:06 |
856 | 4 | 0 | |u http://dx.doi.org/10.3390/v12060647 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 12 |j 2020 |e 6 |b 15 |c 06 |