Identification of a Neutralizing Monoclonal Antibody That Recognizes a Unique Epitope on Domain III of the Envelope Protein of Tembusu Virus

Domain III of the envelope protein (EDIII) is the major target of flavivirus neutralizing antibody. To date, little is known regarding antibody-mediated neutralization of Tembusu virus (TMUV), a novel flavivirus emerging in duck in 2010. Here, a novel monoclonal antibody (MAb), designated 12F11, was prepared by immunization of mice with recombinant EDIII (rEDIII) protein. Using virus neutralization test, 12F11 in undiluted ascites neutralized the TMUV infectivity to induce the development of cytopathic effects in BHK-21 cells, indicating that 12F11 exhibits a neutralizing activity. The neutralizing activity of 12F11 was confirmed by plaque reduction neutralization test, in which 12F11 reduced significantly the number of plaques produced by TMUV in BHK-21 cells. Western blot analyses of a series of truncated rEDIII proteins showed that the epitope recognized by 12F11 includes amino acids between residues 8 and 77 of EDIII protein. Function analysis demonstrated that 12F11 neutralizes TMUV infection at virus adsorption and at a step after adsorption to a certain extent. The present study provides an important step towards elucidating antibody-mediated neutralization of TMUV.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:12

Enthalten in:

Viruses - 12(2020), 6 vom: 15. Juni

Sprache:

Englisch

Beteiligte Personen:

Qu, Shenghua [VerfasserIn]
Wang, Xiaoyan [VerfasserIn]
Yang, Lixin [VerfasserIn]
Lv, Junfeng [VerfasserIn]
Meng, Runze [VerfasserIn]
Dai, Weiqian [VerfasserIn]
Li, Qingxiangzi [VerfasserIn]
Liu, Huicong [VerfasserIn]
Zhang, Bing [VerfasserIn]
Zhang, Dabing [VerfasserIn]

Links:

Volltext

Themen:

Antibodies, Monoclonal
Antibodies, Neutralizing
Antibody-mediated neutralization
Duck
Epitope
Epitopes
Journal Article
Monoclonal antibody
Neutralizing antibody
Research Support, Non-U.S. Gov't
Tembusu virus
Viral Envelope Proteins

Anmerkungen:

Date Completed 19.02.2021

Date Revised 19.02.2021

published: Electronic

Citation Status MEDLINE

doi:

10.3390/v12060647

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311280021