Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch
The detection of dynamic conformational changes in proteins in live cells is challenging. Live-cell FRET (Förster Resonance Energy Transfer) is an example of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure conformational changes in caveolin-1 (Cav-1) oligomers on the surface of plasmalemma vesicles, or caveolae.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2169 |
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Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2169(2020) vom: 16., Seite 71-80 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Zimnicka, Adriana M [VerfasserIn] |
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Links: |
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Themen: |
Cav-1-CFP |
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Anmerkungen: |
Date Completed 31.03.2021 Date Revised 11.01.2023 published: Print Citation Status MEDLINE |
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doi: |
10.1007/978-1-0716-0732-9_7 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM311276067 |
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520 | |a The detection of dynamic conformational changes in proteins in live cells is challenging. Live-cell FRET (Förster Resonance Energy Transfer) is an example of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure conformational changes in caveolin-1 (Cav-1) oligomers on the surface of plasmalemma vesicles, or caveolae | ||
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