Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch

The detection of dynamic conformational changes in proteins in live cells is challenging. Live-cell FRET (Förster Resonance Energy Transfer) is an example of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure conformational changes in caveolin-1 (Cav-1) oligomers on the surface of plasmalemma vesicles, or caveolae.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:2169

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 2169(2020) vom: 16., Seite 71-80

Sprache:

Englisch

Beteiligte Personen:

Zimnicka, Adriana M [VerfasserIn]
Chen, Zhenlong [VerfasserIn]
Toth, Peter T [VerfasserIn]
Minshall, Richard D [VerfasserIn]

Links:

Volltext

Themen:

Cav-1-CFP
Cav-1-YFP
Caveolae
Caveolin 1
Conformation change
Journal Article
Live-cell FRET
Luminescent Proteins

Anmerkungen:

Date Completed 31.03.2021

Date Revised 11.01.2023

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-0716-0732-9_7

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311276067