Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells

© 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd..

AIM: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite - NaOCl (NaOCl/OCT).

METHODOLOGY: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05).

RESULTS: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity.

CONCLUSION: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:53

Enthalten in:

International endodontic journal - 53(2020), 9 vom: 01. Sept., Seite 1264-1274

Sprache:

Englisch

Beteiligte Personen:

Coaguila-Llerena, H [VerfasserIn]
Rodrigues, E M [VerfasserIn]
Santos, C S [VerfasserIn]
Ramos, S G [VerfasserIn]
Medeiros, M C [VerfasserIn]
Chavez-Andrade, G M [VerfasserIn]
Guerreiro-Tanomaru, J M [VerfasserIn]
Tanomaru-Filho, M [VerfasserIn]
Faria, G [VerfasserIn]

Links:

Volltext

Themen:

Cell culture techniques
Chlorhexidine
DY38VHM5OD
Imines
Journal Article
Materials testing
OZE0372S5A
Octenidine
Pyridines
R4KO0DY52L
Root Canal Irrigants
Root canal therapy
Sodium Hypochlorite
Sodium hypochlorite

Anmerkungen:

Date Completed 13.08.2020

Date Revised 04.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/iej.13347

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311150861