Mass spectrometry of urinary β2 microglobulin oligomer in patients with proteinuria
© 2020 International Society for Apheresis, Japanese Society for Apheresis, and Japanese Society for Dialysis Therapy..
Urinary β2 microglobulin (β2-m) is a marker of renal tubule dysfunction; however, β2-m might become degraded under acidic conditions. To confirm the degradation and consequent deactivation of β2-m under acidic conditions, we used matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to detect the levels and forms of β2-m in the urine samples of patients with high proteinuria (n = 21) and healthy subjects (n = 6). β2-m was purified in crude form using immunoprecipitation. A signal of 11.74 kDa, corresponding to the molecular weight of β2-m, was detected in all samples. In addition, several high-molecular-weight proteins were detected in a patient as integrals of the intensity at 11.74 kDa. These results indicate that posttranslational modifications of β2-m might be involved in the pathological process of proteinuria. Therefore, MS can be used for monitoring proteinuria and predicting the risk of progression.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy - 25(2021), 2 vom: 01. Apr., Seite 197-203 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Akizuki, Setsuko [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.10.2021 Date Revised 20.10.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/1744-9987.13547 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311150802 |
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| 520 | |a © 2020 International Society for Apheresis, Japanese Society for Apheresis, and Japanese Society for Dialysis Therapy. | ||
| 520 | |a Urinary β2 microglobulin (β2-m) is a marker of renal tubule dysfunction; however, β2-m might become degraded under acidic conditions. To confirm the degradation and consequent deactivation of β2-m under acidic conditions, we used matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to detect the levels and forms of β2-m in the urine samples of patients with high proteinuria (n = 21) and healthy subjects (n = 6). β2-m was purified in crude form using immunoprecipitation. A signal of 11.74 kDa, corresponding to the molecular weight of β2-m, was detected in all samples. In addition, several high-molecular-weight proteins were detected in a patient as integrals of the intensity at 11.74 kDa. These results indicate that posttranslational modifications of β2-m might be involved in the pathological process of proteinuria. Therefore, MS can be used for monitoring proteinuria and predicting the risk of progression | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) | |
| 650 | 4 | |a oligomer | |
| 650 | 4 | |a posttranslational modification | |
| 650 | 4 | |a urinary β2 microglobulin | |
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| 700 | 1 | |a Kawano, Katsumi |e verfasserin |4 aut | |
| 700 | 1 | |a Iwamoto, Takeo |e verfasserin |4 aut | |
| 700 | 1 | |a Nakada, Koji |e verfasserin |4 aut | |
| 700 | 1 | |a Ohnishi, Akihiro |e verfasserin |4 aut | |
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