Notoginsenoside R1 protects boar sperm during liquid storage at 17°C
© 2020 Blackwell Verlag GmbH..
Reactive oxygen species (ROS) damage mammalian sperm during liquid storage. Notoginsenoside R1 (NR1) is a compound isolated from the roots of Panax notoginseng; it has powerful ROS-scavenging activities. This work hypothesized that the antioxidant capacity of NR1 could improve boar sperm quality and fertility during liquid storage. During liquid storage at 17°C, the supplementation of semen extender with NR1 (50 μM) significantly improved sperm motility, membrane integrity and acrosome integrity after 5 days of preservation. NR1 treatment also reduced ROS and lipid peroxidation (LPO) levels at day 5 (p <0.05). Higher glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and sperm-zona pellucida binding capacity were observed in the 50 μM NR1 group than those in the control group at day 7 (p <0.05). Importantly, statistical analysis of the fertility of 200 sows indicated that addition of NR1 to the extender improved the fertility parameters of boar spermatozoa during liquid storage at 17°C (p <0.05). These results demonstrate the practical feasibility of using 50 μM NR1 as an antioxidant in boar extender during liquid storage at 17°C, which is beneficial to both spermatozoa quality and fertility.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
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| Enthalten in: |
Reproduction in domestic animals = Zuchthygiene - 55(2020), 9 vom: Sept., Seite 1072-1079 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ren, Fa [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.07.2021 Date Revised 15.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/rda.13745 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Reactive oxygen species (ROS) damage mammalian sperm during liquid storage. Notoginsenoside R1 (NR1) is a compound isolated from the roots of Panax notoginseng; it has powerful ROS-scavenging activities. This work hypothesized that the antioxidant capacity of NR1 could improve boar sperm quality and fertility during liquid storage. During liquid storage at 17°C, the supplementation of semen extender with NR1 (50 μM) significantly improved sperm motility, membrane integrity and acrosome integrity after 5 days of preservation. NR1 treatment also reduced ROS and lipid peroxidation (LPO) levels at day 5 (p <0.05). Higher glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and sperm-zona pellucida binding capacity were observed in the 50 μM NR1 group than those in the control group at day 7 (p <0.05). Importantly, statistical analysis of the fertility of 200 sows indicated that addition of NR1 to the extender improved the fertility parameters of boar spermatozoa during liquid storage at 17°C (p <0.05). These results demonstrate the practical feasibility of using 50 μM NR1 as an antioxidant in boar extender during liquid storage at 17°C, which is beneficial to both spermatozoa quality and fertility | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Notoginsenoside R1 | |
| 650 | 4 | |a antioxidant | |
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| 650 | 7 | |a Antioxidants |2 NLM | |
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| 700 | 1 | |a Niu, Tongjuan |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Yitian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Jinhong |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Gaoxiao |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jianhong |e verfasserin |4 aut | |
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