Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System

Plantaricin BM-1, a class IIa bacteriocin produced by Lactobacillus plantarum BM-1, has significant antibacterial activity against Gram-positive and Gram-negative bacteria. This study aimed to explore the role of the Escherichia coli K12 outer membrane (OM) channel protein TolC in the response to plantaricin BM-1. The tolC null mutant (E. coli K12∆tolC) was constructed by Red homologous recombination. The mechanism of tolC regulating the sensitivity of E. coli K12 under plantaricin BM-1 was investigated. tolC null mutation significantly increased the E. coli K12 sensitivity to plantaricin BM-1 and inhibited biofilm formation, and cells ruptured and shrunk. Proteomic analysis showed that the AcrAB-TolC and EmrAB-TolC efflux pumps were significantly (p < 0.05) upregulated in E. coli K12∆tolC. Based on the results of real-time PCR, we concluded that under plantaricin BM-1, the CpxR/CpxA two-component regulatory system of E. coli K12 responded with envelope damage, followed by activation of the transcription of marA and expression of AcrAB-TolC efflux pump. Moreover, tolC null mutation weakened the AcrAB-TolC efflux pump and then increased the sensitivity of E. coli K12 to plantaricin BM-1. These will contribute exploring the action mechanism of class IIa bacteriocins against Gram-negative bacteria.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:13

Enthalten in:

Probiotics and antimicrobial proteins - 13(2021), 1 vom: 11. Feb., Seite 238-248

Sprache:

Englisch

Beteiligte Personen:

Wang, Huan [VerfasserIn]
Zhang, Hongxing [VerfasserIn]
Zhang, Hanwei [VerfasserIn]
Jin, Junhua [VerfasserIn]
Xie, Yuanhong [VerfasserIn]

Links:

Volltext

Themen:

153554-07-5
Bacterial Outer Membrane Proteins
Bacterial Proteins
Bacteriocins
Biofilm
CpxA protein, E coli
CpxR/CpxA two-component
CpxR protein, Bacteria
EC 2.7.-
EC 2.7.3.-
Escherichia coli Proteins
Journal Article
Membrane Transport Proteins
Protein Kinases
Proteome
Research Support, Non-U.S. Gov't
TolC
TolC protein, E coli

Anmerkungen:

Date Completed 16.06.2021

Date Revised 16.06.2021

published: Print

Citation Status MEDLINE

doi:

10.1007/s12602-020-09671-6

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311084656