Thymol and Carvacrol Downregulate the Expression of Salmonella typhimurium Virulence Genes during an In Vitro Infection on Caco-2 Cells

Salmonella typhimurium is one of the major bacteria responsible for gastroenteritis in humans caused by foodborne pathogens. As pork is one of the main routes of transmission, bioactive compounds used as feed additives may be an important strategy to control Salmonella typhimurium. The aim of this study was to assess the antimicrobial activity of several organic acids and nature identical compounds against Salmonella typhimurium ATCC®® 6994™. Moreover, the effect of sub-lethal concentrations of thymol and carvacrol in counteracting a Salmonella typhimurium in vitro infection on Caco-2 cells was evaluated, focusing on the maintenance of the epithelial barrier and the alteration of Salmonella virulence genes. The results showed a protective effect of the compounds on the integrity of the intestinal monolayer, improving transepithelial electrical resistance and bacterial translocation compared to the non-treated cells. A real-time PCR study highlighted a significant downregulation of the main virulence genes of Salmonella (hilA, prgH, invA, sipA, sipC, sipD, sopB, sopE2). These findings indicate that thymol and carvacrol could be good candidates for the control of Salmonella typhimurium in pigs.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:8

Enthalten in:

Microorganisms - 8(2020), 6 vom: 07. Juni

Sprache:

Englisch

Beteiligte Personen:

Giovagnoni, Giulia [VerfasserIn]
Rossi, Barbara [VerfasserIn]
Tugnoli, Benedetta [VerfasserIn]
Ghiselli, Federico [VerfasserIn]
Bonetti, Andrea [VerfasserIn]
Piva, Andrea [VerfasserIn]
Grilli, Ester [VerfasserIn]

Links:

Volltext

Themen:

Antimicrobial activity
Carvacrol
Epithelial barrier
Feed additives
Journal Article
Nature identical compounds
Salmonella typhimurium
Thymol
Tight junctions
Virulence genes

Anmerkungen:

Date Revised 28.09.2020

published: Electronic

Citation Status PubMed-not-MEDLINE

doi:

10.3390/microorganisms8060862

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM310967007